TY - JOUR
T1 - Proficiency of drug susceptibility testing for Mycobacterium tuberculosis in Taiwan, 2007-2011
AU - Wu, M. H.
AU - Chiang, C. Y.
AU - Deng, Y. M.
AU - Wang, T. F.
AU - Jou, Ruwen
PY - 2013/1/1
Y1 - 2013/1/1
N2 - SETTING: Authorised clinical mycobacteriology laboratories in Taiwan. OBJECTIVE: To evaluate the impact of external quality assessment (EQA) on the quality of drug susceptibility testing (DST) in 2007-2011. DESIGN: Panels consisting of 20-30 Mycobacterium tuberculosis strains were used. Efficiency of 95% in detecting resistance to both isoniazid (INH) and rifampicin (RMP), and of 90% to ethambutol (EMB) and streptomycin (SM) was used to define a competent laboratory. RESULTS: The proportion of laboratories that fulfilled the competency criteria for all first-line drugs was 16.7% in 2007, increasing to 85.7% in 2008, 86.1% in 2009, 82.4% in 2010, and to 96.8% in 2011 (P < 0.01). The mean efficiency in detecting resistance to INH and RMP reached >99% during 2008-2011 (P = 0.90 for INH and P = 0.82 for RMP), and for EMB it increased from 82.0% in 2007 to 92.2% in 2008 and 99.5% in 2011 (P < 0.01), while that for resistance to SM increased from 82.0% in 2007 to 98.1% in 2008 and 99.5% in 2011 (P < 0.01). Preparations of inoculum for DST and detection of EMB resistance were the main reasons for non-competence. CONCLUSION: The EQA programme was effective in improving the competency of clinical laboratories in performing DST for tuberculosis.
AB - SETTING: Authorised clinical mycobacteriology laboratories in Taiwan. OBJECTIVE: To evaluate the impact of external quality assessment (EQA) on the quality of drug susceptibility testing (DST) in 2007-2011. DESIGN: Panels consisting of 20-30 Mycobacterium tuberculosis strains were used. Efficiency of 95% in detecting resistance to both isoniazid (INH) and rifampicin (RMP), and of 90% to ethambutol (EMB) and streptomycin (SM) was used to define a competent laboratory. RESULTS: The proportion of laboratories that fulfilled the competency criteria for all first-line drugs was 16.7% in 2007, increasing to 85.7% in 2008, 86.1% in 2009, 82.4% in 2010, and to 96.8% in 2011 (P < 0.01). The mean efficiency in detecting resistance to INH and RMP reached >99% during 2008-2011 (P = 0.90 for INH and P = 0.82 for RMP), and for EMB it increased from 82.0% in 2007 to 92.2% in 2008 and 99.5% in 2011 (P < 0.01), while that for resistance to SM increased from 82.0% in 2007 to 98.1% in 2008 and 99.5% in 2011 (P < 0.01). Preparations of inoculum for DST and detection of EMB resistance were the main reasons for non-competence. CONCLUSION: The EQA programme was effective in improving the competency of clinical laboratories in performing DST for tuberculosis.
KW - Drug susceptibility testing
KW - Mycobacterium tuberculosis
KW - Proficiency
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U2 - 10.5588/ijtld.12.0521
DO - 10.5588/ijtld.12.0521
M3 - Article
C2 - 23232011
AN - SCOPUS:84871210714
SN - 1027-3719
VL - 17
SP - 113-119+i
JO - International Journal of Tuberculosis and Lung Disease
JF - International Journal of Tuberculosis and Lung Disease
IS - 1
ER -