Abstract
The expression of the chemokine, eotaxin-1, and its receptors in normal and osteoarthritic human chondrocytes was examined, and its role in cartilage degradation was elucidated in this study. Results indicated that plasma concentrations of eotaxin-1 as well as the chemokines, RANTES, and MCP-1α, were higher in patients with osteoarthritis (OA) than those in normal humans. Stimulation of chondrocytes with IL-1β or TNF-α significantly induced eotaxin-1 expression. The production of eotaxin-1 induced expression of its own receptor of CCR3 and CCR5 on the cell surface of chondrosarcomas, suggesting that an autocrine/paracrine pathway is involved in eotaxin-1's action. In addition, eotaxin-1 markedly increased the expressions of MMP-3 and MMP-13 mRNA, but had no effect on TIMP-1 expression in chondrocytes. However, pretreatment of anti-eotaxin-1 antibody significantly decreased the MMP-3 expression induced by IL-1β. These results first demonstrate that human chondrocytes express the chemokine, eotaxin-1, and that its expression is induced by treatment with IL-1β and TNF-α. The cytokine-triggered induction of eotaxin-1 further results in enhanced expressions of its own receptor of CCR3, CCR5, and MMPs, suggesting that eotaxin-1 plays an important role in cartilage degradation in OA.
Original language | English |
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Pages (from-to) | 929-939 |
Number of pages | 11 |
Journal | Journal of Cellular Biochemistry |
Volume | 93 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2004 |
Keywords
- Chemokine
- Chondrocyte
- Eotaxin-1
- Matrix metalloproteinase
- Osteoarthritis
ASJC Scopus subject areas
- Biochemistry
- Cell Biology