TY - JOUR
T1 - Production of bacterial cellulose with various additives in a PCS rotating disk bioreactor and its material property analysis
AU - Lin, Shin Ping
AU - Liu, Chi Te
AU - Hsu, Kai Di
AU - Hung, Yu Ting
AU - Shih, Ting Yu
AU - Cheng, Kuan Chen
N1 - Funding Information:
This work was partially sponsored by "Aim for the Top University Plan" 103R7764 of National Taiwan University and the National Science Council, Taiwan, under contract no. 102-2628-B-002-004-MY3. The authors are very grateful to the Joint Center for Instruments and Researches, College of Bioresources and Agriculture, at National Taiwan University and Prof. An-I Yeh from the Graduate Institute of Food Science Technology at National Taiwan University for his assistance with the SEM, X-ray diffraction measurements and tensile strength analysis. The authors would like to thank Tien-Ni Tammy Tseng from the Department of Agricultural Chemistry, National Taiwan University, and Iris Loira Calvar (who is a native speaker) from the Department of Food Technology, Higher Technical School of Agricultural Engineering, Technical University of Madrid, for the English editing.
Publisher Copyright:
© 2016, Springer Science+Business Media Dordrecht.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Previous studies have demonstrated that bacterial cellulose (BC) can be semi-continuously produced by utilizing the plastic composite support-rotating disk bioreactor (PCS-RDB). In this study, different additives, such as microcrystalline cellulose (Avicel was used in this study), carboxymethylcellulose (CMC), agar and sodium alginate, were added to the PCS-RDB culture medium to improve the BC productivity and material properties. The produced BC was then analyzed by Fourier transform infrared spectroscopy (FTIR), scan electron microscopy (SEM), thermogravimetric analysis, X-ray diffraction (XRD) and strength analysis. Adding CMC and Avicel can increase the production of BC in PCS-RDB. The highest BC production reached (0.64 g/slice) when 0.8 % Avicel was added. Data from FTIR, XRD and SEM indicated that CMC and Avicel were incorporated into the BC during production, creating a disordered BC structure and thus reducing crystallinity. Both BCs and additive-altered BCs exhibited similar high water retention abilities (98.6–99 %). Additive-altered BCs exhibit similar strain but lower stress. BC production in PCS-RDB was improved by incorporating different additives, while the material properties of the produced BCs were also modified.
AB - Previous studies have demonstrated that bacterial cellulose (BC) can be semi-continuously produced by utilizing the plastic composite support-rotating disk bioreactor (PCS-RDB). In this study, different additives, such as microcrystalline cellulose (Avicel was used in this study), carboxymethylcellulose (CMC), agar and sodium alginate, were added to the PCS-RDB culture medium to improve the BC productivity and material properties. The produced BC was then analyzed by Fourier transform infrared spectroscopy (FTIR), scan electron microscopy (SEM), thermogravimetric analysis, X-ray diffraction (XRD) and strength analysis. Adding CMC and Avicel can increase the production of BC in PCS-RDB. The highest BC production reached (0.64 g/slice) when 0.8 % Avicel was added. Data from FTIR, XRD and SEM indicated that CMC and Avicel were incorporated into the BC during production, creating a disordered BC structure and thus reducing crystallinity. Both BCs and additive-altered BCs exhibited similar high water retention abilities (98.6–99 %). Additive-altered BCs exhibit similar strain but lower stress. BC production in PCS-RDB was improved by incorporating different additives, while the material properties of the produced BCs were also modified.
KW - Bacterial cellulose
KW - Gluconacetobacter xylinus
KW - Materials property analysis
KW - Plastic composite support
KW - Rotating disk bioreactor
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U2 - 10.1007/s10570-015-0855-0
DO - 10.1007/s10570-015-0855-0
M3 - Article
AN - SCOPUS:84955693638
SN - 0969-0239
VL - 23
SP - 367
EP - 377
JO - Cellulose
JF - Cellulose
IS - 1
ER -