TY - JOUR
T1 - Preparation, physicochemical characterization, and antioxidant effects of quercetin nanoparticles
AU - Wu, Tzu Hui
AU - Yen, Feng Lin
AU - Lin, Liang Tzung
AU - Tsai, Tong Rong
AU - Lin, Chun Ching
AU - Cham, Thau Ming
PY - 2008/1/4
Y1 - 2008/1/4
N2 - The purpose of this study was to develop quercetin-loaded nanoparticles (QUEN) by a nanoprecipitation technique with Eudragit® E (EE) and polyvinyl alcohol (PVA) as carriers, and to evaluate the antioxidant effects of quercetin (QU) and of its nanoparticles. The novel QUEN systems were characterized by particle size and morphology, yield and encapsulation efficiency, differential scanning calorimetry (DSC), powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), 1H nuclear magnetic resonance (1H NMR), and dissolution study. It was observed that the weight ratio of QU:EE:PVA at 1:10:10 carried a particle size of <85 nm, a particle distribution with polydispersity index <0.3, and its yield and encapsulation efficiency were over 99%. The results from XRD and DSC of the QUEN showed that the crystal of the drug might be converted to an amorphous state. The FT-IR and 1H NMR demonstrated that QU formed intermolecular hydrogen bonding with carriers. The release of the drug from the QUEN was 74-fold higher compared with the pure drug. In addition, the antioxidant activity of the QUEN was more effective than pure QU on DPPH scavenging, anti-superoxide formation, superoxide anion scavenging, and anti-lipid peroxidation.
AB - The purpose of this study was to develop quercetin-loaded nanoparticles (QUEN) by a nanoprecipitation technique with Eudragit® E (EE) and polyvinyl alcohol (PVA) as carriers, and to evaluate the antioxidant effects of quercetin (QU) and of its nanoparticles. The novel QUEN systems were characterized by particle size and morphology, yield and encapsulation efficiency, differential scanning calorimetry (DSC), powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), 1H nuclear magnetic resonance (1H NMR), and dissolution study. It was observed that the weight ratio of QU:EE:PVA at 1:10:10 carried a particle size of <85 nm, a particle distribution with polydispersity index <0.3, and its yield and encapsulation efficiency were over 99%. The results from XRD and DSC of the QUEN showed that the crystal of the drug might be converted to an amorphous state. The FT-IR and 1H NMR demonstrated that QU formed intermolecular hydrogen bonding with carriers. The release of the drug from the QUEN was 74-fold higher compared with the pure drug. In addition, the antioxidant activity of the QUEN was more effective than pure QU on DPPH scavenging, anti-superoxide formation, superoxide anion scavenging, and anti-lipid peroxidation.
KW - Anti-lipid peroxidation
KW - Free radical-scavenging activity
KW - H nuclear magnetic resonance
KW - Nanoparticles
KW - Quercetin
UR - http://www.scopus.com/inward/record.url?scp=36048934038&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36048934038&partnerID=8YFLogxK
U2 - 10.1016/j.ijpharm.2007.06.036
DO - 10.1016/j.ijpharm.2007.06.036
M3 - Article
C2 - 17689897
AN - SCOPUS:36048934038
SN - 0378-5173
VL - 346
SP - 160
EP - 168
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
IS - 1-2
ER -