Preparation and characterization of nanoparticles shelled with chitosan for oral insulin delivery

Yu Hsin Lin; Fwu Long Mi; Chiung Tong Chen; Wei Chun Chang; Shu Fen Peng; Hsiang Fa Liang; Hsing Wen Sung

doi:10.1021/bm0607776

Preparation and characterization of nanoparticles shelled with chitosan for oral insulin delivery

Yu Hsin Lin, Fwu Long Mi, Chiung Tong Chen, Wei Chun Chang, Shu Fen Peng, Hsiang Fa Liang, Hsing Wen Sung

Research output: Contribution to journal › Article › peer-review

303 Citations (Scopus)

Abstract

Nanoparticles (NPs) composed of chitosan (CS) and poly(γ-glutamic acid) (γ-PGA) were prepared by a simple ionic-gelation method for oral insulin delivery. Fourier transform infrared (FT-IR) spectra indicated that CS and γ-PGA were ionized at pH 2.5-6.6, while X-ray diffractograms demonstrated that the crystal structure of CS was disrupted after it was combined with γ-PGA. The diameters of the prepared NPs were in the range of 110-150 nm with a negative or positive surface charge, depending on the relative concentrations of CS to γ-PGA used. The NPs with a positive surface charge (or shelled with CS) could transiently open the tight junctions between Caco-2 cells and thus increased the paracellular permeability. After loading of insulin, the NPs remained spherical and the insulin release profiles were significantly affected by their stability in distinct pH environments. The in vivo results clearly indicated that the insulin-loaded NPs could effectively reduce the blood glucose level in a diabetic rat model.

Original language	English
Pages (from-to)	146-152
Number of pages	7
Journal	Biomacromolecules
Volume	8
Issue number	1
DOIs	https://doi.org/10.1021/bm0607776
Publication status	Published - Jan 2007
Externally published	Yes

ASJC Scopus subject areas

Organic Chemistry
Biochemistry, Genetics and Molecular Biology(all)
Polymers and Plastics
Materials Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1021/bm0607776

Cite this

@article{49c6695510d5407495f5cfe9c982c008,

title = "Preparation and characterization of nanoparticles shelled with chitosan for oral insulin delivery",

abstract = "Nanoparticles (NPs) composed of chitosan (CS) and poly(γ-glutamic acid) (γ-PGA) were prepared by a simple ionic-gelation method for oral insulin delivery. Fourier transform infrared (FT-IR) spectra indicated that CS and γ-PGA were ionized at pH 2.5-6.6, while X-ray diffractograms demonstrated that the crystal structure of CS was disrupted after it was combined with γ-PGA. The diameters of the prepared NPs were in the range of 110-150 nm with a negative or positive surface charge, depending on the relative concentrations of CS to γ-PGA used. The NPs with a positive surface charge (or shelled with CS) could transiently open the tight junctions between Caco-2 cells and thus increased the paracellular permeability. After loading of insulin, the NPs remained spherical and the insulin release profiles were significantly affected by their stability in distinct pH environments. The in vivo results clearly indicated that the insulin-loaded NPs could effectively reduce the blood glucose level in a diabetic rat model.",

author = "Lin, {Yu Hsin} and Mi, {Fwu Long} and Chen, {Chiung Tong} and Chang, {Wei Chun} and Peng, {Shu Fen} and Liang, {Hsiang Fa} and Sung, {Hsing Wen}",

year = "2007",

month = jan,

doi = "10.1021/bm0607776",

language = "English",

volume = "8",

pages = "146--152",

journal = "Biomacromolecules",

issn = "1525-7797",

publisher = "American Chemical Society",

number = "1",

}

TY - JOUR

T1 - Preparation and characterization of nanoparticles shelled with chitosan for oral insulin delivery

AU - Lin, Yu Hsin

AU - Mi, Fwu Long

AU - Chen, Chiung Tong

AU - Chang, Wei Chun

AU - Peng, Shu Fen

AU - Liang, Hsiang Fa

AU - Sung, Hsing Wen

PY - 2007/1

Y1 - 2007/1

N2 - Nanoparticles (NPs) composed of chitosan (CS) and poly(γ-glutamic acid) (γ-PGA) were prepared by a simple ionic-gelation method for oral insulin delivery. Fourier transform infrared (FT-IR) spectra indicated that CS and γ-PGA were ionized at pH 2.5-6.6, while X-ray diffractograms demonstrated that the crystal structure of CS was disrupted after it was combined with γ-PGA. The diameters of the prepared NPs were in the range of 110-150 nm with a negative or positive surface charge, depending on the relative concentrations of CS to γ-PGA used. The NPs with a positive surface charge (or shelled with CS) could transiently open the tight junctions between Caco-2 cells and thus increased the paracellular permeability. After loading of insulin, the NPs remained spherical and the insulin release profiles were significantly affected by their stability in distinct pH environments. The in vivo results clearly indicated that the insulin-loaded NPs could effectively reduce the blood glucose level in a diabetic rat model.

AB - Nanoparticles (NPs) composed of chitosan (CS) and poly(γ-glutamic acid) (γ-PGA) were prepared by a simple ionic-gelation method for oral insulin delivery. Fourier transform infrared (FT-IR) spectra indicated that CS and γ-PGA were ionized at pH 2.5-6.6, while X-ray diffractograms demonstrated that the crystal structure of CS was disrupted after it was combined with γ-PGA. The diameters of the prepared NPs were in the range of 110-150 nm with a negative or positive surface charge, depending on the relative concentrations of CS to γ-PGA used. The NPs with a positive surface charge (or shelled with CS) could transiently open the tight junctions between Caco-2 cells and thus increased the paracellular permeability. After loading of insulin, the NPs remained spherical and the insulin release profiles were significantly affected by their stability in distinct pH environments. The in vivo results clearly indicated that the insulin-loaded NPs could effectively reduce the blood glucose level in a diabetic rat model.

UR - http://www.scopus.com/inward/record.url?scp=33846410236&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846410236&partnerID=8YFLogxK

U2 - 10.1021/bm0607776

DO - 10.1021/bm0607776

M3 - Article

C2 - 17206800

AN - SCOPUS:33846410236

SN - 1525-7797

VL - 8

SP - 146

EP - 152

JO - Biomacromolecules

JF - Biomacromolecules

IS - 1

ER -

Preparation and characterization of nanoparticles shelled with chitosan for oral insulin delivery

Abstract

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this