TY - JOUR
T1 - Preparation and characterization of moldable demineralized bone matrix/calcium sulfate composite bone graft materials
AU - Chen, I. Cheng
AU - Su, Chen Ying
AU - Lai, Chun Cheih
AU - Tsou, Yi Syue
AU - Zheng, Yudong
AU - Fang, Hsu Wei
N1 - Funding Information:
Funding: This work was supported by National Taipei University of Technology and University of Science and Technology Beijing Joint Research Program, NTUT-USTB-108-09.
Publisher Copyright:
© 2021 Academic Center for Education, Culture and Research TMU.
PY - 2021/12
Y1 - 2021/12
N2 - Demineralized bone matrix (DBM) is a decalcified allo/xenograft retaining collagen and noncollagenous proteins, which has been extensively used because of its osteoconductive and osteoinductive properties. Calcium sulfate (CaSO4, CS) is a synthetic bone substitute used in bone healing with biocompatible, nontoxic, bioabsorbable, osteoconductive, and good mechanical characteristics. This study aims to prepare a DBM/CS composite bone graft material in a moldable putty form without compromising the peculiar properties of DBM and CS. For this purpose, firstly, porcine femur was defatted using chloroform/methanol and extracted by acid for demineralization, then freeze-dried and milled/sieved to obtain DBM powder. Secondly, the α-form and β-form of calcium sulfate hemihydrate (CaSO4 0.5H2O, CSH) were produced by heating gypsum (CaSO4 2H2O). The morphology and particle sizes of α- and β-CSH were obtained by SEM, and their chemical properties were confirmed by EDS, FTIR and XRD. Furthermore, the DBM-based graft was mixed with α-or β-CSH at a ratio of 9:1, and glycerol/4% HPMC was added as a carrier to produce a putty. DBM/CSH putty possesses a low washout rate, good mechanical strength and biocompatibility. In conclusion, we believe that the moldable DBM/CSH composite putty developed in this study could be a promising substitute for the currently available bone grafts, and might have practical application in the orthopedics field as a potential bone void filler.
AB - Demineralized bone matrix (DBM) is a decalcified allo/xenograft retaining collagen and noncollagenous proteins, which has been extensively used because of its osteoconductive and osteoinductive properties. Calcium sulfate (CaSO4, CS) is a synthetic bone substitute used in bone healing with biocompatible, nontoxic, bioabsorbable, osteoconductive, and good mechanical characteristics. This study aims to prepare a DBM/CS composite bone graft material in a moldable putty form without compromising the peculiar properties of DBM and CS. For this purpose, firstly, porcine femur was defatted using chloroform/methanol and extracted by acid for demineralization, then freeze-dried and milled/sieved to obtain DBM powder. Secondly, the α-form and β-form of calcium sulfate hemihydrate (CaSO4 0.5H2O, CSH) were produced by heating gypsum (CaSO4 2H2O). The morphology and particle sizes of α- and β-CSH were obtained by SEM, and their chemical properties were confirmed by EDS, FTIR and XRD. Furthermore, the DBM-based graft was mixed with α-or β-CSH at a ratio of 9:1, and glycerol/4% HPMC was added as a carrier to produce a putty. DBM/CSH putty possesses a low washout rate, good mechanical strength and biocompatibility. In conclusion, we believe that the moldable DBM/CSH composite putty developed in this study could be a promising substitute for the currently available bone grafts, and might have practical application in the orthopedics field as a potential bone void filler.
KW - Bioceramics
KW - Biocomposites
KW - Bone implants
KW - Calcium sulfate
KW - DBM
KW - Demineralized bone matrix
KW - Moldable
KW - Putty
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U2 - 10.3390/jfb12040056
DO - 10.3390/jfb12040056
M3 - Article
AN - SCOPUS:85118138606
SN - 2079-4983
VL - 12
JO - Journal of Functional Biomaterials
JF - Journal of Functional Biomaterials
IS - 4
M1 - 56
ER -