TEMPO-oxidization and mechanical disintegration were utilized to develop sacchachitin nanofibers (SCNF) with a 3D gel structure for being an ideal scaffold. Mechanically disintegrated SCNF (MDSCNF) with NanoLyzer® at 20,000 psi for 5 cycles and TEMPO-oxidized SCNF (TOSCNF) produced with 5.0 and 10.0 mmole NaClO/g SC was designated as SCN5, T050SC, and T100SC, respectively. All 2% MDSCNF suspensions were demonstrated to be in gel form, while all except T100SC of 2% TOSCNF suspensions showed to be wet fiber-like hydrogel. In diabetic wound healing study, both SCN5 and T050SC incorporated in AMPS (2-acrylamide-2-methyl-propane sulfonate)-based wound dressing were showed to accelerate diabetic wound healing forming nearly the same as normal tissues. T050SC/H further provided the healed wound with growth of sweat glands and hair follicles indicating the wound had healed as functional tissue. Conclusively, TEMPO-oxidized SCNF-based hydrogel scaffolds showed greater potentials in tissue regeneration due to its unique physical and chemical properties.

Original languageEnglish
Article number115507
Pages (from-to)115507
JournalCarbohydrate Polymers
Publication statusPublished - Feb 1 2020


  • 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO)
  • 2-acrylamido-2-methyl-1-propanesulfonic acid sodium salt (AMPS)
  • Chitosan
  • Diabetic wound healing
  • Irgacure® 184
  • L-ascorbic acid, poly(ethylene glycol) diacrylate
  • Mechanical disintegration
  • N,N-dimethylacrylamide
  • Nanofibers
  • PEG1000
  • PVP K90
  • Sacchachitin
  • Sodium bromide
  • Sodium hypochlorite
  • TEMPO-oxidation
  • Thiazolyl blue tetrazolium bromide (MTT reagent)
  • chitosan
  • sodium bromide
  • thiazolyl blue tetrazolium bromide (MTT reagent)

ASJC Scopus subject areas

  • Materials Chemistry
  • Polymers and Plastics
  • Organic Chemistry


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