Preliminary crystallographic studies of the Fab fragment of an anti-azophenylarsonate antibody

Shin Fuw Lin; Miquel Coll; Malcolm L. Gefter; Michael N. Margolies; Andrew H.J. Wang

doi:10.1016/0022-2836(89)90587-1

Preliminary crystallographic studies of the Fab fragment of an anti-azophenylarsonate antibody

Shin Fuw Lin, Miquel Coll, Malcolm L. Gefter, Michael N. Margolies, Andrew H.J. Wang

Research output: Contribution to journal › Article › peer-review

Abstract

Single crystals of the Fab fragment of a murine A/J anti-azophenylarsonate monoclonal antibody have been prepared by the vapor diffusion method. Antibody 3A7 uses the same combination of variable region gene segments (V_K, J_K, V_H, J_H) as do antizophenylarsonate antibodies bearing a predominant cross-reactive idiotype, but utilizes a different D gene segment. The crystals grow in the presence of β-octylglucoside as tetragonal bipyramids in the space group of either P4₁2₁2 or P4₃3₁2 and with unit cell dimensions of a = b = 77·9 A ̊, and c = 146·7 A ̊. They diffract X-rays to better than 2·7 Å resolution. Data up to 2·7 Å resolution have been collected.

Original language	English
Pages (from-to)	789-790
Number of pages	2
Journal	Journal of Molecular Biology
Volume	206
Issue number	4
DOIs	https://doi.org/10.1016/0022-2836(89)90587-1
Publication status	Published - Apr 20 1989
Externally published	Yes

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1016/0022-2836(89)90587-1

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title = "Preliminary crystallographic studies of the Fab fragment of an anti-azophenylarsonate antibody",

abstract = "Single crystals of the Fab fragment of a murine A/J anti-azophenylarsonate monoclonal antibody have been prepared by the vapor diffusion method. Antibody 3A7 uses the same combination of variable region gene segments (VK, JK, VH, JH) as do antizophenylarsonate antibodies bearing a predominant cross-reactive idiotype, but utilizes a different D gene segment. The crystals grow in the presence of β-octylglucoside as tetragonal bipyramids in the space group of either P41212 or P43312 and with unit cell dimensions of a = b = 77·9 A ̊, and c = 146·7 A ̊. They diffract X-rays to better than 2·7 {\AA} resolution. Data up to 2·7 {\AA} resolution have been collected.",

author = "Lin, {Shin Fuw} and Miquel Coll and Gefter, {Malcolm L.} and Margolies, {Michael N.} and Wang, {Andrew H.J.}",

note = "Funding Information: This work was supported by the National Institutes of Health (GM 37641 and CA 24432). M.C. is a Fulbright fellow.",

year = "1989",

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doi = "10.1016/0022-2836(89)90587-1",

language = "English",

volume = "206",

pages = "789--790",

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publisher = "Academic Press Inc.",

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T1 - Preliminary crystallographic studies of the Fab fragment of an anti-azophenylarsonate antibody

AU - Lin, Shin Fuw

AU - Coll, Miquel

AU - Gefter, Malcolm L.

AU - Margolies, Michael N.

AU - Wang, Andrew H.J.

N1 - Funding Information: This work was supported by the National Institutes of Health (GM 37641 and CA 24432). M.C. is a Fulbright fellow.

PY - 1989/4/20

Y1 - 1989/4/20

N2 - Single crystals of the Fab fragment of a murine A/J anti-azophenylarsonate monoclonal antibody have been prepared by the vapor diffusion method. Antibody 3A7 uses the same combination of variable region gene segments (VK, JK, VH, JH) as do antizophenylarsonate antibodies bearing a predominant cross-reactive idiotype, but utilizes a different D gene segment. The crystals grow in the presence of β-octylglucoside as tetragonal bipyramids in the space group of either P41212 or P43312 and with unit cell dimensions of a = b = 77·9 A ̊, and c = 146·7 A ̊. They diffract X-rays to better than 2·7 Å resolution. Data up to 2·7 Å resolution have been collected.

AB - Single crystals of the Fab fragment of a murine A/J anti-azophenylarsonate monoclonal antibody have been prepared by the vapor diffusion method. Antibody 3A7 uses the same combination of variable region gene segments (VK, JK, VH, JH) as do antizophenylarsonate antibodies bearing a predominant cross-reactive idiotype, but utilizes a different D gene segment. The crystals grow in the presence of β-octylglucoside as tetragonal bipyramids in the space group of either P41212 or P43312 and with unit cell dimensions of a = b = 77·9 A ̊, and c = 146·7 A ̊. They diffract X-rays to better than 2·7 Å resolution. Data up to 2·7 Å resolution have been collected.

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Preliminary crystallographic studies of the Fab fragment of an anti-azophenylarsonate antibody

Abstract

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