Preliminary crystallographic studies of the Fab fragment of an anti-azophenylarsonate antibody

Shin Fuw Lin, Miquel Coll, Malcolm L. Gefter, Michael N. Margolies, Andrew H.J. Wang

Research output: Contribution to journalArticlepeer-review

Abstract

Single crystals of the Fab fragment of a murine A/J anti-azophenylarsonate monoclonal antibody have been prepared by the vapor diffusion method. Antibody 3A7 uses the same combination of variable region gene segments (VK, JK, VH, JH) as do antizophenylarsonate antibodies bearing a predominant cross-reactive idiotype, but utilizes a different D gene segment. The crystals grow in the presence of β-octylglucoside as tetragonal bipyramids in the space group of either P41212 or P43312 and with unit cell dimensions of a = b = 77·9 A ̊, and c = 146·7 A ̊. They diffract X-rays to better than 2·7 Å resolution. Data up to 2·7 Å resolution have been collected.

Original languageEnglish
Pages (from-to)789-790
Number of pages2
JournalJournal of Molecular Biology
Volume206
Issue number4
DOIs
Publication statusPublished - Apr 20 1989
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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