TY - JOUR
T1 - Potential role of the src gene product in inhibition of gap-junctional communication in NIH/3T3 cells
AU - Chang, C. C.
AU - Trosko, J. E.
AU - Kung, H. J.
AU - Bombick, D.
AU - Matsumura, F.
PY - 1985/11/6
Y1 - 1985/11/6
N2 - The effects of the src gene on the activity of protein kinase C and intercellular communication have been studied in transformed NIH/3T3 clones isolated from soft agar following transfection with the plasmid carrying the v-src gene (p(src-11)). Six transformed clones that were studied contained newly incorporated v-src genes in the genome, had an increased amount of pp60(src), and showed enhanced activities of protein kinase C. Intercellular communication, studied by observing with autoradiography the transfer of [3H]uridine nucleotide from prelabeled donor cells to recipient cells in contact, was found to be reduced in transformed clones as compared to parental NIH/3T3 cells. Treatment with phorbol 12-myristate 13-acetate was also found to increase protein kinase C activity and to reduce intercellular communication in normal NIH/3T3 cells. These results suggest that the v-src gene product, in a manner similar to some of the powerful tumor promoters, may directly or indirectly affect cell-cell communication.
AB - The effects of the src gene on the activity of protein kinase C and intercellular communication have been studied in transformed NIH/3T3 clones isolated from soft agar following transfection with the plasmid carrying the v-src gene (p(src-11)). Six transformed clones that were studied contained newly incorporated v-src genes in the genome, had an increased amount of pp60(src), and showed enhanced activities of protein kinase C. Intercellular communication, studied by observing with autoradiography the transfer of [3H]uridine nucleotide from prelabeled donor cells to recipient cells in contact, was found to be reduced in transformed clones as compared to parental NIH/3T3 cells. Treatment with phorbol 12-myristate 13-acetate was also found to increase protein kinase C activity and to reduce intercellular communication in normal NIH/3T3 cells. These results suggest that the v-src gene product, in a manner similar to some of the powerful tumor promoters, may directly or indirectly affect cell-cell communication.
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U2 - 10.1073/pnas.82.16.5360
DO - 10.1073/pnas.82.16.5360
M3 - Article
C2 - 2991919
AN - SCOPUS:0346804737
SN - 0027-8424
VL - 82
SP - 5360
EP - 5364
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 16
ER -