Portland cement induces human periodontal ligament cells to differentiate by upregulating miR-146a

Min Ching Wang, Li Yin Yeh, Wen Yu Shih, Wan Chun Li, Kuo Wei Chang, Shu Chun Lin

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Background/Purpose: Bioaggregates such as Portland cement (PC) can be an economical alternative for mineral trioxide aggregate (MTA) with additional benefit of less discoloration. MTA has been known to induce differentiations of several dental cells. MicroRNAs are important regulators of biological processes, including differentiation, physiologic homeostasis, and disease progression. This study is to explore how PC enhances the differentiation of periodontal ligament (PDL) cells in microRNAs level. Methods: PDL cells were cultured in a regular PC- or MTA-conditioned medium or an osteoinduction medium (OIM). Alizarin red staining was used to evaluate the extent of mineralization. Transfection of microRNA mimics induced exogenous miR-31 and miR-146a expression. The expression of microRNAs and differentiation markers was assayed using reverse-transcriptase polymerase chain reaction. Results: PC enhanced the mineralization of PDL cells in a dose-dependent manner in the OIM. Exogenous miR-31 and miR-146a expression upregulated alkaline phosphatase (ALP), bone morphogenic protein (BMP), and dentin matrix protein 1 (DMP1) expression. However, miR-31 and miR-146a modulates cementum protein 1 (CEMP1) expression in different ways. PC also enhanced ALP and BMP but attenuated CEMP1 in the OIM. Although the OIM or PC treatment upregulated miR-21, miR-29b, and miR-146a, only miR-146a was able to be induced by PC in combination with OIM. Conclusion: This study demonstrated that PC enhances the differentiation of PDL cells, especially osteogenic through miR-146a upregulation. In order to control the ankylosis after regenerative endodontics with the usage of bioaggregates, further investigations to explore these differentiation mechanisms in the miRNA level may be needed.

Original languageEnglish
Pages (from-to)308-315
Number of pages8
JournalJournal of the Formosan Medical Association
Issue number4
Publication statusPublished - Apr 2018
Externally publishedYes


  • Bioaggregate
  • miR-146a
  • Osteogenic differentiation
  • Periodontal ligament (PDL)
  • Portland cement

ASJC Scopus subject areas

  • General Medicine


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