Polymeric micelle gene delivery of bcl-xL via eye drop reduced corneal apoptosis following epithelial debridement

Yaw Chong Tong; Shwu Fen Chang; Winston W.Y. Kao; Chia Yang Liu; Jiahorng Liaw

doi:10.1016/j.jconrel.2010.06.006

Polymeric micelle gene delivery of bcl-x_L via eye drop reduced corneal apoptosis following epithelial debridement

Yaw Chong Tong, Shwu Fen Chang, Winston W.Y. Kao, Chia Yang Liu, Jiahorng Liaw

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Stromal keratocyte apoptosis triggered by epithelial injury is one mechanism of corneal disorders. A model of epithelial injury by epithelial debridement is established, and keratocyte apoptosis is evidenced by DNA fragmentation and cellular morphological changes in the anterior stroma underlying the injured epithelium. Delivery of plasmid (pCMV-bcl-x_L-eGFP) encoding an anti-apoptotic gene, the bcl-x_L with a nano-carrier, polymeric micelles (PM) via eye drop to cornea after epithelial debridement, the mRNA level of bcl-x_L was significantly increased (2.2-fold, P<0.05) at 48h and the eGFP mRNA was detected (4571.7±1194.5copies/μg total RNA). The bcl-x_L-eGFP fusion protein was also detected in wounded cornea at 48h after delivery, accompanying with decreased DNA fragmentation and lower caspase-3 activity (P<0.05). In conclusion, eye drop of pCMV-bcl-x_L-eGFP/PM reduced corneal apoptosis following epithelial debridement.

Original language	English
Pages (from-to)	76-83
Number of pages	8
Journal	Journal of Controlled Release
Volume	147
Issue number	1
DOIs	https://doi.org/10.1016/j.jconrel.2010.06.006
Publication status	Published - Oct 2010

Keywords

Apoptosis
Bcl-x
Cornea
Eye drop
Polymeric micelle

ASJC Scopus subject areas

Pharmaceutical Science

Access to Document

10.1016/j.jconrel.2010.06.006

Cite this

@article{91feaf5679304e1e934ad4095174b0b0,

title = "Polymeric micelle gene delivery of bcl-xL via eye drop reduced corneal apoptosis following epithelial debridement",

abstract = "Stromal keratocyte apoptosis triggered by epithelial injury is one mechanism of corneal disorders. A model of epithelial injury by epithelial debridement is established, and keratocyte apoptosis is evidenced by DNA fragmentation and cellular morphological changes in the anterior stroma underlying the injured epithelium. Delivery of plasmid (pCMV-bcl-xL-eGFP) encoding an anti-apoptotic gene, the bcl-xL with a nano-carrier, polymeric micelles (PM) via eye drop to cornea after epithelial debridement, the mRNA level of bcl-xL was significantly increased (2.2-fold, P<0.05) at 48h and the eGFP mRNA was detected (4571.7±1194.5copies/μg total RNA). The bcl-xL-eGFP fusion protein was also detected in wounded cornea at 48h after delivery, accompanying with decreased DNA fragmentation and lower caspase-3 activity (P<0.05). In conclusion, eye drop of pCMV-bcl-xL-eGFP/PM reduced corneal apoptosis following epithelial debridement.",

keywords = "Apoptosis, Bcl-x, Cornea, Eye drop, Polymeric micelle",

author = "Tong, {Yaw Chong} and Chang, {Shwu Fen} and Kao, {Winston W.Y.} and Liu, {Chia Yang} and Jiahorng Liaw",

year = "2010",

month = oct,

doi = "10.1016/j.jconrel.2010.06.006",

language = "English",

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journal = "Journal of Controlled Release",

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T1 - Polymeric micelle gene delivery of bcl-xL via eye drop reduced corneal apoptosis following epithelial debridement

AU - Tong, Yaw Chong

AU - Chang, Shwu Fen

AU - Kao, Winston W.Y.

AU - Liu, Chia Yang

AU - Liaw, Jiahorng

PY - 2010/10

Y1 - 2010/10

N2 - Stromal keratocyte apoptosis triggered by epithelial injury is one mechanism of corneal disorders. A model of epithelial injury by epithelial debridement is established, and keratocyte apoptosis is evidenced by DNA fragmentation and cellular morphological changes in the anterior stroma underlying the injured epithelium. Delivery of plasmid (pCMV-bcl-xL-eGFP) encoding an anti-apoptotic gene, the bcl-xL with a nano-carrier, polymeric micelles (PM) via eye drop to cornea after epithelial debridement, the mRNA level of bcl-xL was significantly increased (2.2-fold, P<0.05) at 48h and the eGFP mRNA was detected (4571.7±1194.5copies/μg total RNA). The bcl-xL-eGFP fusion protein was also detected in wounded cornea at 48h after delivery, accompanying with decreased DNA fragmentation and lower caspase-3 activity (P<0.05). In conclusion, eye drop of pCMV-bcl-xL-eGFP/PM reduced corneal apoptosis following epithelial debridement.

AB - Stromal keratocyte apoptosis triggered by epithelial injury is one mechanism of corneal disorders. A model of epithelial injury by epithelial debridement is established, and keratocyte apoptosis is evidenced by DNA fragmentation and cellular morphological changes in the anterior stroma underlying the injured epithelium. Delivery of plasmid (pCMV-bcl-xL-eGFP) encoding an anti-apoptotic gene, the bcl-xL with a nano-carrier, polymeric micelles (PM) via eye drop to cornea after epithelial debridement, the mRNA level of bcl-xL was significantly increased (2.2-fold, P<0.05) at 48h and the eGFP mRNA was detected (4571.7±1194.5copies/μg total RNA). The bcl-xL-eGFP fusion protein was also detected in wounded cornea at 48h after delivery, accompanying with decreased DNA fragmentation and lower caspase-3 activity (P<0.05). In conclusion, eye drop of pCMV-bcl-xL-eGFP/PM reduced corneal apoptosis following epithelial debridement.

KW - Apoptosis

KW - Bcl-x

KW - Cornea

KW - Eye drop

KW - Polymeric micelle

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