Polyamide thin-layer chromatography of phosphorylated tyrosine, threonine, and serine

Wen Chang Chang; Muh Lin Lee; Chen Kung Chou; Sheng Chung Lee

doi:10.1016/0003-2697(83)90018-0

Polyamide thin-layer chromatography of phosphorylated tyrosine, threonine, and serine

Wen Chang Chang, Muh Lin Lee, Chen Kung Chou, Sheng Chung Lee

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

One-dimensional thin-layer chromatography on polyamide plates offers an easy and rapid identification of O-phosphotyrosine. The thin-layer plate is developed for 30 min in 5% propionic acid containing 0.013%-0.025% sodium dodecyl sulfate. O-Phosphotyrosine, with R_f = 0.54, can be well separated from O-phosphothreonine and O-phosphoserine, which comigrate at R_f = 0.72.

Original language	English
Pages (from-to)	342-344
Number of pages	3
Journal	Analytical Biochemistry
Volume	132
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(83)90018-0
Publication status	Published - Jul 15 1983
Externally published	Yes

Keywords

phosphoamino acids
polyamide
TLC

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(83)90018-0

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title = "Polyamide thin-layer chromatography of phosphorylated tyrosine, threonine, and serine",

abstract = "One-dimensional thin-layer chromatography on polyamide plates offers an easy and rapid identification of O-phosphotyrosine. The thin-layer plate is developed for 30 min in 5% propionic acid containing 0.013%-0.025% sodium dodecyl sulfate. O-Phosphotyrosine, with Rf = 0.54, can be well separated from O-phosphothreonine and O-phosphoserine, which comigrate at Rf = 0.72.",

keywords = "phosphoamino acids, polyamide, TLC",

author = "Chang, {Wen Chang} and Lee, {Muh Lin} and Chou, {Chen Kung} and Lee, {Sheng Chung}",

year = "1983",

month = jul,

day = "15",

doi = "10.1016/0003-2697(83)90018-0",

language = "English",

volume = "132",

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journal = "Analytical Biochemistry",

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T1 - Polyamide thin-layer chromatography of phosphorylated tyrosine, threonine, and serine

AU - Chang, Wen Chang

AU - Lee, Muh Lin

AU - Chou, Chen Kung

AU - Lee, Sheng Chung

PY - 1983/7/15

Y1 - 1983/7/15

N2 - One-dimensional thin-layer chromatography on polyamide plates offers an easy and rapid identification of O-phosphotyrosine. The thin-layer plate is developed for 30 min in 5% propionic acid containing 0.013%-0.025% sodium dodecyl sulfate. O-Phosphotyrosine, with Rf = 0.54, can be well separated from O-phosphothreonine and O-phosphoserine, which comigrate at Rf = 0.72.

AB - One-dimensional thin-layer chromatography on polyamide plates offers an easy and rapid identification of O-phosphotyrosine. The thin-layer plate is developed for 30 min in 5% propionic acid containing 0.013%-0.025% sodium dodecyl sulfate. O-Phosphotyrosine, with Rf = 0.54, can be well separated from O-phosphothreonine and O-phosphoserine, which comigrate at Rf = 0.72.

KW - phosphoamino acids

KW - polyamide

KW - TLC

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UR - http://www.scopus.com/inward/citedby.url?scp=0020517188&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(83)90018-0

DO - 10.1016/0003-2697(83)90018-0

M3 - Article

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AN - SCOPUS:0020517188

SN - 0003-2697

VL - 132

SP - 342

EP - 344

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Polyamide thin-layer chromatography of phosphorylated tyrosine, threonine, and serine

Abstract

Keywords

ASJC Scopus subject areas

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