TY - JOUR
T1 - Platonin preserves blood-brain barrier integrity in septic rats
AU - Yeh, Chia Tse
AU - Kao, Ming Chang
AU - Chen, Cay Huyen
AU - Huang, Chun Jen
N1 - Publisher Copyright:
Copyright © 2015, Taiwan Society of Anesthesiologists. Published by Elsevier Taiwan LLC. All rights reserved.
PY - 2015/3/1
Y1 - 2015/3/1
N2 - Objectives Platonin possesses potent anti-inflammatory and antioxidative capacities. Because systemic inflammation and oxidative stress are crucial in mediating sepsis-induced blood-brain barrier (BBB) integrity loss, this study elucidated the effects of platonin on preserving BBB integrity in septic rats. Methods A total of 72 adult male rats (200-250 g) were randomized to receive cecal ligation and puncture (CLP), CLP plus platonin, sham operation, or sham operation plus platonin (n = 18 in each group). Systemic inflammation and oxidation levels and BBB integrity in the surviving rats were determined after 24-hour monitoring. Results Plasma levels of interleukin-6 (IL-6) and malondialdehyde (MDA) - markers of systemic inflammation and oxidation - and the grading of Evans blue staining of the brains, BBB permeability to Evans blue dye, and brain edema levels - markers of BBB integrity - in rats that received CLP were significantly higher than rats that received sham operation (all p < 0.001). By contrast, the plasma levels of IL-6 (p < 0.001) and MDA (p < 0.001), and the grading of Evans blue staining (p = 0.015), BBB permeability to Evans blue dye (p = 0.043), and brain edema levels (p = 0.034) in rats that received CLP plus platonin were significantly lower than rats that received CLP. Experimental data further revealed that the concentration of tight junction protein claudin-5, a major structural component of BBB, in rats that received CLP was significantly lower than rats that received CLP plus platonin (p = 0.023). Conclusion Platonin could attenuate sepsis-induced BBB integrity loss in rats.
AB - Objectives Platonin possesses potent anti-inflammatory and antioxidative capacities. Because systemic inflammation and oxidative stress are crucial in mediating sepsis-induced blood-brain barrier (BBB) integrity loss, this study elucidated the effects of platonin on preserving BBB integrity in septic rats. Methods A total of 72 adult male rats (200-250 g) were randomized to receive cecal ligation and puncture (CLP), CLP plus platonin, sham operation, or sham operation plus platonin (n = 18 in each group). Systemic inflammation and oxidation levels and BBB integrity in the surviving rats were determined after 24-hour monitoring. Results Plasma levels of interleukin-6 (IL-6) and malondialdehyde (MDA) - markers of systemic inflammation and oxidation - and the grading of Evans blue staining of the brains, BBB permeability to Evans blue dye, and brain edema levels - markers of BBB integrity - in rats that received CLP were significantly higher than rats that received sham operation (all p < 0.001). By contrast, the plasma levels of IL-6 (p < 0.001) and MDA (p < 0.001), and the grading of Evans blue staining (p = 0.015), BBB permeability to Evans blue dye (p = 0.043), and brain edema levels (p = 0.034) in rats that received CLP plus platonin were significantly lower than rats that received CLP. Experimental data further revealed that the concentration of tight junction protein claudin-5, a major structural component of BBB, in rats that received CLP was significantly lower than rats that received CLP plus platonin (p = 0.023). Conclusion Platonin could attenuate sepsis-induced BBB integrity loss in rats.
KW - cecal ligation and puncture
KW - Evans blue dye
KW - inflammation
KW - rat
KW - tight junction
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U2 - 10.1016/j.aat.2015.02.001
DO - 10.1016/j.aat.2015.02.001
M3 - Article
C2 - 25791838
AN - SCOPUS:84928626842
SN - 1875-4597
VL - 53
SP - 12
EP - 15
JO - Acta Anaesthesiologica Taiwanica
JF - Acta Anaesthesiologica Taiwanica
IS - 1
ER -