Platelets stimulate aortic smooth muscle cell migration in vitro. Involvement of 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid

Junko Nakao; Toshiro Ooyama; Wen Chang Chang; Sei itsu Murota; Hajime Orimo

doi:10.1016/0021-9150(82)90018-1

Platelets stimulate aortic smooth muscle cell migration in vitro. Involvement of 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid

Junko Nakao, Toshiro Ooyama, Wen Chang Chang, Sei itsu Murota, Hajime Orimo

Research output: Contribution to journal › Article › peer-review

72 Citations (Scopus)

Abstract

The migration of rat aortic smooth muscle cells was measured in modified Boyden chambers. Smooth muscle cells were motile in vitro and their migration was stimulated (time- and dose-dependently) by a platelet-derived factor. Treatment of platelets with indomethacin resulted in a significant increase in smooth muscle cell migration, whereas treatment with 5,8,11,14-eicosatetraenoic acid inhibited it. Purified 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid at a very low concentration (6 × 10^-15-6 ×^-13 g/ml) significantly stimulated smooth muscle cell migration. The locomotion induced by 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid was chemokinetic. These findings point to the physiological importance of a platelet 12-lipoxygenase product of arachidonic acid in the early phase of atherosclerosis.

Original language	English
Pages (from-to)	143-150
Number of pages	8
Journal	Atherosclerosis
Volume	43
Issue number	2-3
DOIs	https://doi.org/10.1016/0021-9150(82)90018-1
Publication status	Published - Jun 1982
Externally published	Yes

Keywords

12-l-Hydroxy-5,8,10,14-eicosatetraenoic acid
Atherosclerosis
Chemokinesis
Platelet
Smooth muscle cell migration

ASJC Scopus subject areas

Cardiology and Cardiovascular Medicine

Access to Document

10.1016/0021-9150(82)90018-1

Cite this

@article{d5d32d05b9b54f669be29ec235b8df81,

title = "Platelets stimulate aortic smooth muscle cell migration in vitro. Involvement of 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid",

abstract = "The migration of rat aortic smooth muscle cells was measured in modified Boyden chambers. Smooth muscle cells were motile in vitro and their migration was stimulated (time- and dose-dependently) by a platelet-derived factor. Treatment of platelets with indomethacin resulted in a significant increase in smooth muscle cell migration, whereas treatment with 5,8,11,14-eicosatetraenoic acid inhibited it. Purified 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid at a very low concentration (6 × 10-15-6 ×-13 g/ml) significantly stimulated smooth muscle cell migration. The locomotion induced by 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid was chemokinetic. These findings point to the physiological importance of a platelet 12-lipoxygenase product of arachidonic acid in the early phase of atherosclerosis.",

keywords = "12-l-Hydroxy-5,8,10,14-eicosatetraenoic acid, Atherosclerosis, Chemokinesis, Platelet, Smooth muscle cell migration",

author = "Junko Nakao and Toshiro Ooyama and Chang, {Wen Chang} and Murota, {Sei itsu} and Hajime Orimo",

year = "1982",

month = jun,

doi = "10.1016/0021-9150(82)90018-1",

language = "English",

volume = "43",

pages = "143--150",

journal = "Atherosclerosis",

issn = "0021-9150",

publisher = "Elsevier Ireland Ltd",

number = "2-3",

}

TY - JOUR

T1 - Platelets stimulate aortic smooth muscle cell migration in vitro. Involvement of 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid

AU - Nakao, Junko

AU - Ooyama, Toshiro

AU - Chang, Wen Chang

AU - Murota, Sei itsu

AU - Orimo, Hajime

PY - 1982/6

Y1 - 1982/6

N2 - The migration of rat aortic smooth muscle cells was measured in modified Boyden chambers. Smooth muscle cells were motile in vitro and their migration was stimulated (time- and dose-dependently) by a platelet-derived factor. Treatment of platelets with indomethacin resulted in a significant increase in smooth muscle cell migration, whereas treatment with 5,8,11,14-eicosatetraenoic acid inhibited it. Purified 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid at a very low concentration (6 × 10-15-6 ×-13 g/ml) significantly stimulated smooth muscle cell migration. The locomotion induced by 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid was chemokinetic. These findings point to the physiological importance of a platelet 12-lipoxygenase product of arachidonic acid in the early phase of atherosclerosis.

AB - The migration of rat aortic smooth muscle cells was measured in modified Boyden chambers. Smooth muscle cells were motile in vitro and their migration was stimulated (time- and dose-dependently) by a platelet-derived factor. Treatment of platelets with indomethacin resulted in a significant increase in smooth muscle cell migration, whereas treatment with 5,8,11,14-eicosatetraenoic acid inhibited it. Purified 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid at a very low concentration (6 × 10-15-6 ×-13 g/ml) significantly stimulated smooth muscle cell migration. The locomotion induced by 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid was chemokinetic. These findings point to the physiological importance of a platelet 12-lipoxygenase product of arachidonic acid in the early phase of atherosclerosis.

KW - 12-l-Hydroxy-5,8,10,14-eicosatetraenoic acid

KW - Atherosclerosis

KW - Chemokinesis

KW - Platelet

KW - Smooth muscle cell migration

UR - http://www.scopus.com/inward/record.url?scp=0019946806&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019946806&partnerID=8YFLogxK

U2 - 10.1016/0021-9150(82)90018-1

DO - 10.1016/0021-9150(82)90018-1

M3 - Article

C2 - 7115458

AN - SCOPUS:0019946806

SN - 0021-9150

VL - 43

SP - 143

EP - 150

JO - Atherosclerosis

JF - Atherosclerosis

IS - 2-3

ER -

Platelets stimulate aortic smooth muscle cell migration in vitro. Involvement of 12-l-hydroxy-5,8,10,14-eicosatetraenoic acid

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this