TY - JOUR
T1 - Platelet-rich fibrin facilitates one-stage cartilage repair by promoting chondrocytes viability, migration, and matrix synthesis
AU - Wong, Chin Chean
AU - Ou, Keng Liang
AU - Lin, Yun Ho
AU - Lin, Ming Fang
AU - Yang, Tsung Lin
AU - Chen, Chih Hwa
AU - Chan, Wing P.
N1 - Funding Information:
Acknowledgments: This study was supported in part by a grant from the Taiwan Ministry of Science and Technology (MOST 108-2314-B-038-131) and Taipei Medical University (IIT-1072-1). The authors are grateful to Yao-Hung Wang for performing anesthesia and Yu-Hao Chan for coordinating the details of the experiment. The authors also thank Jin-Hua Chen and Ching-Wen Tsai from the Biostatistics Center of Taipei Medical University for statistical analysis.
Funding Information:
This study was supported in part by a grant from the Taiwan Ministry of Science and Technology (MOST 108-2314-B-038-131) and Taipei Medical University (IIT-1072-1). The authors are grateful to Yao-Hung Wang for performing anesthesia and Yu-Hao Chan for coordinating the details of the experiment. The authors also thank Jin-Hua Chen and Ching-Wen Tsai from the Biostatistics Center of Taipei Medical University for statistical analysis.
PY - 2020/1/2
Y1 - 2020/1/2
N2 - The main aim of this study is to develop a one-stage method to combine platelet-rich fibrin (PRF) and autologous cartilage autografts for porcine articular cartilage repair. The porcine chondrocytes were treated with different concentrations of PRF-conditioned media and were evaluated for their cell viability and extracellular glycosaminoglycan (GAG) synthesis during six day cultivation. The chemotactic effects of PRF on chondrocytes on undigested cartilage autografts were revealed in explant cultures. For the in vivo part, porcine chondral defects were created at the medial femoral condyles of which were (1) left untreated, (2) implanted with PRF combined with hand-diced cartilage grafts, or (3) implanted with PRF combined with device-diced cartilage grafts. After six months, gross grades, histological, and immunohistochemical analyses were compared. The results showed that PRF promotes the viability and GAG expression of the cultured chondrocytes. Additionally, the PRF-conditioned media induce significant cellular migration and outgrowth of chondrocytes from undigested cartilage grafts. In the in vivo study, gross grading and histological scores showed significantly better outcomes in the treatment groups as compared with controls. Moreover, both treatment groups showed significantly more type II collagen staining and minimal type I collagen staining as compared with controls, indicating more hyaline-like cartilage and less fibrous tissue. In conclusion, PRF enhances the viability, differentiation, and migration of chondrocytes, thus, showing an appealing capacity for cartilage repair. The data altogether provide evidences to confirm the feasibility of a one-stage, culture-free method of combining PRF and cartilage autografts for repairing articular cartilage defects. From translational standpoints, these advantages benefit clinical applications by simplifying and potentiating the efficacy of cartilage autograft transplants.
AB - The main aim of this study is to develop a one-stage method to combine platelet-rich fibrin (PRF) and autologous cartilage autografts for porcine articular cartilage repair. The porcine chondrocytes were treated with different concentrations of PRF-conditioned media and were evaluated for their cell viability and extracellular glycosaminoglycan (GAG) synthesis during six day cultivation. The chemotactic effects of PRF on chondrocytes on undigested cartilage autografts were revealed in explant cultures. For the in vivo part, porcine chondral defects were created at the medial femoral condyles of which were (1) left untreated, (2) implanted with PRF combined with hand-diced cartilage grafts, or (3) implanted with PRF combined with device-diced cartilage grafts. After six months, gross grades, histological, and immunohistochemical analyses were compared. The results showed that PRF promotes the viability and GAG expression of the cultured chondrocytes. Additionally, the PRF-conditioned media induce significant cellular migration and outgrowth of chondrocytes from undigested cartilage grafts. In the in vivo study, gross grading and histological scores showed significantly better outcomes in the treatment groups as compared with controls. Moreover, both treatment groups showed significantly more type II collagen staining and minimal type I collagen staining as compared with controls, indicating more hyaline-like cartilage and less fibrous tissue. In conclusion, PRF enhances the viability, differentiation, and migration of chondrocytes, thus, showing an appealing capacity for cartilage repair. The data altogether provide evidences to confirm the feasibility of a one-stage, culture-free method of combining PRF and cartilage autografts for repairing articular cartilage defects. From translational standpoints, these advantages benefit clinical applications by simplifying and potentiating the efficacy of cartilage autograft transplants.
KW - Autografts
KW - Cartilage
KW - Chondrocytes
KW - Knee
KW - Platelet-rich fibrin (PRF)
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UR - http://www.scopus.com/inward/citedby.url?scp=85078080300&partnerID=8YFLogxK
U2 - 10.3390/ijms21020577
DO - 10.3390/ijms21020577
M3 - Article
C2 - 31963217
AN - SCOPUS:85078080300
SN - 1661-6596
VL - 21
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 2
M1 - 577
ER -