Physiological and functional interactions between Tcf4 and Daxx in colon cancer cells

Shu Ling Tzeng, Yu Wen Cheng, Ching Hao Li, Young Sun Lin, Hey Chi Hsu, Jaw Jou Kang

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


Daxx, a human cell death-associated protein, was isolated as a Tcf4-interacting protein, using a yeast two-hybrid screen. Co- immunoprecipitation in HEK-293T cells and yeast two-hybrid screen in Y190 cells were performed to identify the interaction between Tcf4 with Daxx and to map the binding regions of Tcf4. In the nucleus, Daxx reduced DNA binding activity of Tcf4 and repressed Tcf4 transcriptional activity. Overexpression of Daxx altered the expression of genes downstream of Tcf4, including cyclin D1 and Hath-1, and induced G1 phase arrest in colon cancer cells. A reduction in Daxx protein expression was also observed in colon adenocarcinoma tissue when compared with normal colon tissue. This evidence suggests a possible physiological function of Daxx, via interaction with Tcf4, to regulate proliferation and differentiation of colon cells.

Original languageEnglish
Pages (from-to)15405-15411
Number of pages7
JournalJournal of Biological Chemistry
Issue number22
Publication statusPublished - Jun 2 2006
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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