Physical and functional interactions between hnRNP K and PRMT family proteins

James Yi Hsin Chan; Tsai Yuan Hsieh; Shu Ting Liu; Wei Yuan Chou; Min Huey Chung; Shih Ming Huang

doi:10.1016/j.febslet.2008.12.025

Physical and functional interactions between hnRNP K and PRMT family proteins

James Yi Hsin Chan, Tsai Yuan Hsieh, Shu Ting Liu, Wei Yuan Chou, Min Huey Chung, Shih Ming Huang

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

The mechanism underlying the protein-protein interaction of hnRNP K and PRMT family proteins is unclear. We examined and confirmed the arginine methylation of hnRNP K protein by PRMT1, not CARM1, via their direct binding. We also studied hnRNP K protein complexes containing CARM1, as well as PRMT1, using co-immunoprecipitation analysis. PRMT family proteins might be involved in the regulation of hnRNP K functions in nuclear receptor coactivator, transactivation, and p21 gene and protein expressions. We believe these observations will help provide insights into the regulation of hnRNP K protein functions via the recruitment of its associated proteins, including its arginine methylation-modifying proteins. Structured summary: MINT-6803853:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT1 (uniprotkb:Q99873) by pull down (MI:0096). MINT-6803884:. hnRPK, (uniprotkb:P61978) physically interacts (MI:0218) with CARM1 (uniprotkb:Q86X55) by anti tag coimmunoprecipitation (MI:0007). MINT-6803869:. hnRPK, (uniprotkb:P61978) physically interacts (MI:0218) with PRMT1 (uniprotkb:Q99873) by anti tag coimmunoprecipitation (MI:0007). MINT-6803939:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT2 (uniprotkb:P55345) by pull down (MI:0096). MINT-6803929:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to RMT (uniprotkb:P38074) by pull down (MI:0096). MINT-6803896:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT3 (uniprotkb:O60678) by pull down (MI:0096). MINT-6803834:. PRMT1 (uniprotkb:Q99873) methylates (MI:0213) hnRPK, (uniprotkb:P61978) by methyltransferase assay (MI:0515).

Original language	English
Pages (from-to)	281-286
Number of pages	6
Journal	FEBS Letters
Volume	583
Issue number	2
DOIs	https://doi.org/10.1016/j.febslet.2008.12.025
Publication status	Published - Jan 22 2009

Keywords

HCV Core protein
hnRNP K
PRMT
Protein-protein interaction

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2008.12.025

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@article{281cf0fd2f39409ca595480ea0d7edca,

title = "Physical and functional interactions between hnRNP K and PRMT family proteins",

abstract = "The mechanism underlying the protein-protein interaction of hnRNP K and PRMT family proteins is unclear. We examined and confirmed the arginine methylation of hnRNP K protein by PRMT1, not CARM1, via their direct binding. We also studied hnRNP K protein complexes containing CARM1, as well as PRMT1, using co-immunoprecipitation analysis. PRMT family proteins might be involved in the regulation of hnRNP K functions in nuclear receptor coactivator, transactivation, and p21 gene and protein expressions. We believe these observations will help provide insights into the regulation of hnRNP K protein functions via the recruitment of its associated proteins, including its arginine methylation-modifying proteins. Structured summary: MINT-6803853:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT1 (uniprotkb:Q99873) by pull down (MI:0096). MINT-6803884:. hnRPK, (uniprotkb:P61978) physically interacts (MI:0218) with CARM1 (uniprotkb:Q86X55) by anti tag coimmunoprecipitation (MI:0007). MINT-6803869:. hnRPK, (uniprotkb:P61978) physically interacts (MI:0218) with PRMT1 (uniprotkb:Q99873) by anti tag coimmunoprecipitation (MI:0007). MINT-6803939:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT2 (uniprotkb:P55345) by pull down (MI:0096). MINT-6803929:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to RMT (uniprotkb:P38074) by pull down (MI:0096). MINT-6803896:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT3 (uniprotkb:O60678) by pull down (MI:0096). MINT-6803834:. PRMT1 (uniprotkb:Q99873) methylates (MI:0213) hnRPK, (uniprotkb:P61978) by methyltransferase assay (MI:0515).",

keywords = "HCV Core protein, hnRNP K, PRMT, Protein-protein interaction",

author = "Chan, {James Yi Hsin} and Hsieh, {Tsai Yuan} and Liu, {Shu Ting} and Chou, {Wei Yuan} and Chung, {Min Huey} and Huang, {Shih Ming}",

year = "2009",

month = jan,

day = "22",

doi = "10.1016/j.febslet.2008.12.025",

language = "English",

volume = "583",

pages = "281--286",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "2",

}

TY - JOUR

T1 - Physical and functional interactions between hnRNP K and PRMT family proteins

AU - Chan, James Yi Hsin

AU - Hsieh, Tsai Yuan

AU - Liu, Shu Ting

AU - Chou, Wei Yuan

AU - Chung, Min Huey

AU - Huang, Shih Ming

PY - 2009/1/22

Y1 - 2009/1/22

N2 - The mechanism underlying the protein-protein interaction of hnRNP K and PRMT family proteins is unclear. We examined and confirmed the arginine methylation of hnRNP K protein by PRMT1, not CARM1, via their direct binding. We also studied hnRNP K protein complexes containing CARM1, as well as PRMT1, using co-immunoprecipitation analysis. PRMT family proteins might be involved in the regulation of hnRNP K functions in nuclear receptor coactivator, transactivation, and p21 gene and protein expressions. We believe these observations will help provide insights into the regulation of hnRNP K protein functions via the recruitment of its associated proteins, including its arginine methylation-modifying proteins. Structured summary: MINT-6803853:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT1 (uniprotkb:Q99873) by pull down (MI:0096). MINT-6803884:. hnRPK, (uniprotkb:P61978) physically interacts (MI:0218) with CARM1 (uniprotkb:Q86X55) by anti tag coimmunoprecipitation (MI:0007). MINT-6803869:. hnRPK, (uniprotkb:P61978) physically interacts (MI:0218) with PRMT1 (uniprotkb:Q99873) by anti tag coimmunoprecipitation (MI:0007). MINT-6803939:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT2 (uniprotkb:P55345) by pull down (MI:0096). MINT-6803929:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to RMT (uniprotkb:P38074) by pull down (MI:0096). MINT-6803896:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT3 (uniprotkb:O60678) by pull down (MI:0096). MINT-6803834:. PRMT1 (uniprotkb:Q99873) methylates (MI:0213) hnRPK, (uniprotkb:P61978) by methyltransferase assay (MI:0515).

AB - The mechanism underlying the protein-protein interaction of hnRNP K and PRMT family proteins is unclear. We examined and confirmed the arginine methylation of hnRNP K protein by PRMT1, not CARM1, via their direct binding. We also studied hnRNP K protein complexes containing CARM1, as well as PRMT1, using co-immunoprecipitation analysis. PRMT family proteins might be involved in the regulation of hnRNP K functions in nuclear receptor coactivator, transactivation, and p21 gene and protein expressions. We believe these observations will help provide insights into the regulation of hnRNP K protein functions via the recruitment of its associated proteins, including its arginine methylation-modifying proteins. Structured summary: MINT-6803853:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT1 (uniprotkb:Q99873) by pull down (MI:0096). MINT-6803884:. hnRPK, (uniprotkb:P61978) physically interacts (MI:0218) with CARM1 (uniprotkb:Q86X55) by anti tag coimmunoprecipitation (MI:0007). MINT-6803869:. hnRPK, (uniprotkb:P61978) physically interacts (MI:0218) with PRMT1 (uniprotkb:Q99873) by anti tag coimmunoprecipitation (MI:0007). MINT-6803939:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT2 (uniprotkb:P55345) by pull down (MI:0096). MINT-6803929:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to RMT (uniprotkb:P38074) by pull down (MI:0096). MINT-6803896:. hnRPK, (uniprotkb:P61978) binds (MI:0407) to PRMT3 (uniprotkb:O60678) by pull down (MI:0096). MINT-6803834:. PRMT1 (uniprotkb:Q99873) methylates (MI:0213) hnRPK, (uniprotkb:P61978) by methyltransferase assay (MI:0515).

KW - HCV Core protein

KW - hnRNP K

KW - PRMT

KW - Protein-protein interaction

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U2 - 10.1016/j.febslet.2008.12.025

DO - 10.1016/j.febslet.2008.12.025

M3 - Article

C2 - 19101556

AN - SCOPUS:58249097456

SN - 0014-5793

VL - 583

SP - 281

EP - 286

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Physical and functional interactions between hnRNP K and PRMT family proteins

Abstract

Keywords

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