Photoreactivity of 5-lodouracil-containing DNA-Sso7d complex in solution: The protein-induced DNA kink causes intrastrand hydrogen abstraction from the 5-methyl of thymine at the 5′ side

Takanori Oyoshi; Andrew H.J. Wang; Hiroshi Sugiyama

doi:10.1021/ja016968s

Photoreactivity of 5-lodouracil-containing DNA-Sso7d complex in solution: The protein-induced DNA kink causes intrastrand hydrogen abstraction from the 5-methyl of thymine at the 5′ side

Takanori Oyoshi, Andrew H.J. Wang, Hiroshi Sugiyama

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Photoirradiation of 5-iodouracil-containing DNA, d(GTAAT^|UAC)₂ with Sso7d protein, possessing significant kink in DNA in the crystal structure induces an unprecedented intrastrand H abstraction at the methyl group of T₅, together with selective photooxidations at Met29 of Sso7d. The reactivity of the deoxyuridin-5-yl radical can be explained by the crystal structure of the d(GTAATTAC)₂-Sso7d complex, suggesting that the interaction of DNA-Sso7d in solution is substantially similar to its crystal structure.

Original language	English
Pages (from-to)	2086-2087
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	124
Issue number	10
DOIs	https://doi.org/10.1021/ja016968s
Publication status	Published - Mar 13 2002
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja016968s

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Photoreactivity of 5-lodouracil-containing DNA-Sso7d complex in solution: The protein-induced DNA kink causes intrastrand hydrogen abstraction from the 5-methyl of thymine at the 5′ side. / Oyoshi, Takanori; Wang, Andrew H.J.; Sugiyama, Hiroshi.
In: Journal of the American Chemical Society, Vol. 124, No. 10, 13.03.2002, p. 2086-2087.

Research output: Contribution to journal › Article › peer-review

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abstract = "Photoirradiation of 5-iodouracil-containing DNA, d(GTAAT|UAC)2 with Sso7d protein, possessing significant kink in DNA in the crystal structure induces an unprecedented intrastrand H abstraction at the methyl group of T5, together with selective photooxidations at Met29 of Sso7d. The reactivity of the deoxyuridin-5-yl radical can be explained by the crystal structure of the d(GTAATTAC)2-Sso7d complex, suggesting that the interaction of DNA-Sso7d in solution is substantially similar to its crystal structure.",

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AB - Photoirradiation of 5-iodouracil-containing DNA, d(GTAAT|UAC)2 with Sso7d protein, possessing significant kink in DNA in the crystal structure induces an unprecedented intrastrand H abstraction at the methyl group of T5, together with selective photooxidations at Met29 of Sso7d. The reactivity of the deoxyuridin-5-yl radical can be explained by the crystal structure of the d(GTAATTAC)2-Sso7d complex, suggesting that the interaction of DNA-Sso7d in solution is substantially similar to its crystal structure.

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Photoreactivity of 5-lodouracil-containing DNA-Sso7d complex in solution: The protein-induced DNA kink causes intrastrand hydrogen abstraction from the 5-methyl of thymine at the 5′ side

Abstract

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