TY - GEN
T1 - Peritoneal Tumor Microenvironment Labchip For The Selection Of Hipec Drugs
AU - Chien, Yi Shan
AU - Wang, Chia Peng
AU - Hsieh, Mao Chih
AU - Tai, Yu Ting
AU - Chi, Jen Tsan Ashley
AU - Don, Si Jin
AU - Liu, Cheng Hsien
N1 - Publisher Copyright:
© 2023 IEEJ.
PY - 2023
Y1 - 2023
N2 - Cancer is the leading cause of death worldwide, and therapies to overcome cancer metastasis and cell proliferation are challenging. Hyperthermic Intraperitoneal Chemotherapy (HIPEC) is often a last resort. Our goal is to develop a platform for HIPEC drug screening. We have fabricated a three-layer microfluidic chip with specific channels designed for the automatic combination of drugs. Additionally, cancer cells and fibroblasts are arrayed to mimic the actual tumor microenvironment. Hydrogel and collagen were used for cell attachment, and drugs flowed directly to the cell culture chamber. During the experiment, the operating temperature was maintained at 43 . Finally, cells were stained with the LIVE/DEAD assay to observe cell viability. Based on the experimental results, the combined effect of two drugs is higher than that of single drug conditions. The combination of Docetaxel and Mitomycin C had the most significant impact among the various drug tests used in this research study.
AB - Cancer is the leading cause of death worldwide, and therapies to overcome cancer metastasis and cell proliferation are challenging. Hyperthermic Intraperitoneal Chemotherapy (HIPEC) is often a last resort. Our goal is to develop a platform for HIPEC drug screening. We have fabricated a three-layer microfluidic chip with specific channels designed for the automatic combination of drugs. Additionally, cancer cells and fibroblasts are arrayed to mimic the actual tumor microenvironment. Hydrogel and collagen were used for cell attachment, and drugs flowed directly to the cell culture chamber. During the experiment, the operating temperature was maintained at 43 . Finally, cells were stained with the LIVE/DEAD assay to observe cell viability. Based on the experimental results, the combined effect of two drugs is higher than that of single drug conditions. The combination of Docetaxel and Mitomycin C had the most significant impact among the various drug tests used in this research study.
KW - Dielectrophoresis
KW - Drug testing platform
KW - Hyperthermic Intraperitoneal Chemotherapy
KW - Microfluidics
UR - http://www.scopus.com/inward/record.url?scp=85193476234&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85193476234&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85193476234
T3 - 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023
SP - 1024
EP - 1027
BT - 2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023
Y2 - 25 June 2023 through 29 June 2023
ER -
