Perilla citriodora from Taiwan and its phytochemical characteristics

Michiho Ito; Fumiyuki Kiuchi; Ling Ling Yang; Gisho Honda

doi:10.1248/bpb.23.359

Perilla citriodora from Taiwan and its phytochemical characteristics

Michiho Ito, Fumiyuki Kiuchi, Ling Ling Yang, Gisho Honda

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

PerilLa citriodora NAKAI, the wild species of Perilia, was collected from Taiwan and its essential oil analyzed. GC-MS analysis of its oil showed that it has a novel composition of limonene (23.5%) and elemicin (17.8%). In Japanese Perilia, a monoterpene (limonene) and a phenylpropanoid (elemicin) have not been detected in the same plant. To compare the sequence similarity of a secondary metabolic enzyme between P. frutescens and P. citriodora, the nucleic acid sequence of the limonene synthase in this P. citriodora was analyzed using the reverse transcript-polymerase chain reaction (RT-PCR) method. Primers for PCR were designed by employing the known sequence of the limonene synthase cloned from P. frutescens. It was found that the limonene synthase in P. citriodora and that in P. frutescens share a high sequence identity, probably indicating that both enzymes evolved from a common ancestor.

Original language	English
Pages (from-to)	359-362
Number of pages	4
Journal	Biological and Pharmaceutical Bulletin
Volume	23
Issue number	3
DOIs	https://doi.org/10.1248/bpb.23.359
Publication status	Published - Mar 2000

Keywords

GC-MS
Limonene synthase
Perilla citriodora
Sequence analysis

ASJC Scopus subject areas

Molecular Medicine
Pharmacology, Toxicology and Pharmaceutics(all)

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title = "Perilla citriodora from Taiwan and its phytochemical characteristics",

abstract = "PerilLa citriodora NAKAI, the wild species of Perilia, was collected from Taiwan and its essential oil analyzed. GC-MS analysis of its oil showed that it has a novel composition of limonene (23.5%) and elemicin (17.8%). In Japanese Perilia, a monoterpene (limonene) and a phenylpropanoid (elemicin) have not been detected in the same plant. To compare the sequence similarity of a secondary metabolic enzyme between P. frutescens and P. citriodora, the nucleic acid sequence of the limonene synthase in this P. citriodora was analyzed using the reverse transcript-polymerase chain reaction (RT-PCR) method. Primers for PCR were designed by employing the known sequence of the limonene synthase cloned from P. frutescens. It was found that the limonene synthase in P. citriodora and that in P. frutescens share a high sequence identity, probably indicating that both enzymes evolved from a common ancestor.",

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author = "Michiho Ito and Fumiyuki Kiuchi and Yang, {Ling Ling} and Gisho Honda",

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AU - Kiuchi, Fumiyuki

AU - Yang, Ling Ling

AU - Honda, Gisho

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Y1 - 2000/3

N2 - PerilLa citriodora NAKAI, the wild species of Perilia, was collected from Taiwan and its essential oil analyzed. GC-MS analysis of its oil showed that it has a novel composition of limonene (23.5%) and elemicin (17.8%). In Japanese Perilia, a monoterpene (limonene) and a phenylpropanoid (elemicin) have not been detected in the same plant. To compare the sequence similarity of a secondary metabolic enzyme between P. frutescens and P. citriodora, the nucleic acid sequence of the limonene synthase in this P. citriodora was analyzed using the reverse transcript-polymerase chain reaction (RT-PCR) method. Primers for PCR were designed by employing the known sequence of the limonene synthase cloned from P. frutescens. It was found that the limonene synthase in P. citriodora and that in P. frutescens share a high sequence identity, probably indicating that both enzymes evolved from a common ancestor.

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KW - GC-MS

KW - Limonene synthase

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Perilla citriodora from Taiwan and its phytochemical characteristics

Abstract

Keywords

ASJC Scopus subject areas

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