TY - JOUR
T1 - PCL/Graphene Scaffolds for the Osteogenesis Process
AU - Anitasari, Silvia
AU - Wu, Ching Zong
AU - Shen, Yung Kang
N1 - Funding Information:
The authors would like to acknowledge to the Ministry of Science and Technology, Taiwan, which funded the project through the project No. MOST 111-2221-E-038-021.
Publisher Copyright:
© 2023 by the authors.
PY - 2023/3
Y1 - 2023/3
N2 - This study aims to characterize the osteoconductivity, optimal bioresorbable, biodegradability, biocompatibility, and mechanical properties of Poly-ε-caprolactone (PCL)/graphene (G) scaffolds at concentrations of 0.5, 1, 1.5, 2, 2.5, and 3 wt%, which are used to support bone regeneration through solvent casting and particulate leaching. The water contact angle measurement revealed a transition from a hydrophobic to a hydrophilic surface after incorporating various G concentrations. The scaffolds with 0.5 wt% G had smaller pores compared to those produced using 3 wt% G. Furthermore, numerous pores were connected, particularly those with larger diameters in the 2 and 3 wt% G samples. The proportion of water absorption varied between 50% and 350% for 4 months, with large percentages of scaffolds containing high G concentrations. Raman spectroscopy and X-ray diffraction, which were used to confirm the presence of nanofiller by increasing the ratios of ID/IG, I2D/IG, and band 2θ = 26.48°. The mechanical properties were improved by the addition of G, with a Young’s modulus of 3 wt% G, four times that of PCL. Measuring cell biocompatibility, adhesion, proliferation, and differentiation with osteoblast-like (MG-63) cells revealed that PCL/G scaffolds with higher concentrations were more biocompatible than PCL as well as those with lower concentrations.
AB - This study aims to characterize the osteoconductivity, optimal bioresorbable, biodegradability, biocompatibility, and mechanical properties of Poly-ε-caprolactone (PCL)/graphene (G) scaffolds at concentrations of 0.5, 1, 1.5, 2, 2.5, and 3 wt%, which are used to support bone regeneration through solvent casting and particulate leaching. The water contact angle measurement revealed a transition from a hydrophobic to a hydrophilic surface after incorporating various G concentrations. The scaffolds with 0.5 wt% G had smaller pores compared to those produced using 3 wt% G. Furthermore, numerous pores were connected, particularly those with larger diameters in the 2 and 3 wt% G samples. The proportion of water absorption varied between 50% and 350% for 4 months, with large percentages of scaffolds containing high G concentrations. Raman spectroscopy and X-ray diffraction, which were used to confirm the presence of nanofiller by increasing the ratios of ID/IG, I2D/IG, and band 2θ = 26.48°. The mechanical properties were improved by the addition of G, with a Young’s modulus of 3 wt% G, four times that of PCL. Measuring cell biocompatibility, adhesion, proliferation, and differentiation with osteoblast-like (MG-63) cells revealed that PCL/G scaffolds with higher concentrations were more biocompatible than PCL as well as those with lower concentrations.
KW - biocompatible
KW - biodegradable
KW - graphene
KW - PCL
KW - scaffold
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U2 - 10.3390/bioengineering10030305
DO - 10.3390/bioengineering10030305
M3 - Article
AN - SCOPUS:85151615584
VL - 10
JO - Bioengineering
JF - Bioengineering
IS - 3
M1 - 305
ER -