Parallel alignment of bacteria using near-field optical force array for cell sorting

H. T. Zhao, Y. Zhang, L. K. Chin, P. H. Yap, K. Wang, W. Ser, A. Q. Liu

Research output: Chapter in Book/Report/Conference proceedingConference contribution


This paper presents a near-field approach to align multiple rod-shaped bacteria based on the interference pattern in silicon nano-waveguide arrays. The bacteria in the optical field will be first trapped by the gradient force and then rotated by the scattering force to the equilibrium position. In the experiment, the Shigella bacteria is rotated 90 deg and aligned to horizontal direction in 9.4 s. Meanwhile, ∼150 Shigella is trapped on the surface in 5 min and 86% is aligned with angle <; 5 deg. This method is a promising toolbox for the research of parallel single-cell biophysical characterization, cell-cell interaction, etc.

Original languageEnglish
Title of host publicationOptical Trapping and Optical Micromanipulation XIV
EditorsGabriel C. Spalding, Kishan Dholakia
ISBN (Electronic)9781510611511
Publication statusPublished - Jan 1 2017
EventOptical Trapping and Optical Micromanipulation XIV 2017 - San Diego, United States
Duration: Aug 6 2017Aug 10 2017

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


ConferenceOptical Trapping and Optical Micromanipulation XIV 2017
Country/TerritoryUnited States
CitySan Diego


  • align
  • near-field
  • Rod-Shaped bacteria
  • trap

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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