TY - JOUR
T1 - Panoramic Tissue Examination That Integrates 3-Dimensional Pathology Imaging and Gene Mutation
T2 - Potential Utility in Non-Small Cell Lung Cancer
AU - Wang, Lei-Chi
AU - Hsieh, Yu-Han
AU - Hung, Yu-Ling
AU - Jiang, Yu-Ting
AU - Lin, Yu-Chieh
AU - Chang, Margaret Dah-Tsyr
AU - Lin, Yen-Yin
AU - Chou, Teh-Ying
N1 - Copyright © 2023 United States & Canadian Academy of Pathology. Published by Elsevier Inc. All rights reserved.
PY - 2023/9
Y1 - 2023/9
N2 - Novel therapeutics have significantly improved the survival and quality of life of patients with malignancies in this century. Versatile precision diagnostic data were used to formulate personalized therapeutic strategies for patients. However, the cost of extensive information depends on the consumption of the specimen, raising the challenges of effective specimen utilization, particularly in small biopsies. In this study, we proposed a tissue-processing cascaded protocol that obtains 3-dimensional (3D) protein expression spatial distribution and mutation analysis from an identical specimen. In order to reuse the thick section tissue evaluated after the 3D pathology technique, we designed a novel high-flatness agarose-embedded method that could improve tissue utilization rate by 1.52 fold, whereas it reduced the tissue-processing time by 80% compared with the traditional paraffin-embedding method. In animal studies, we demonstrated that the protocol would not affect the results of DNA mutation analysis. Furthermore, we explored the utility of this approach in non-small cell lung cancer because it is a compelling application for this innovation. We used 35 cases including 7 cases of biopsy specimens of non-small cell lung cancer to simulate future clinical application. The cascaded protocol consumed 150-μm thickness of formalin-fixed, paraffin-embedded specimens, providing 3D histologic and immunohistochemical information approximately 38 times that of the current paraffin-embedding protocol, and 3 rounds of DNA mutation analysis, offering both essential guidance for routine diagnostic evaluation and advanced information for precision medicine. Our designed integrated workflow provides an alternative way for pathological examination and paves the way for multidimensional tumor tissue assessment.
AB - Novel therapeutics have significantly improved the survival and quality of life of patients with malignancies in this century. Versatile precision diagnostic data were used to formulate personalized therapeutic strategies for patients. However, the cost of extensive information depends on the consumption of the specimen, raising the challenges of effective specimen utilization, particularly in small biopsies. In this study, we proposed a tissue-processing cascaded protocol that obtains 3-dimensional (3D) protein expression spatial distribution and mutation analysis from an identical specimen. In order to reuse the thick section tissue evaluated after the 3D pathology technique, we designed a novel high-flatness agarose-embedded method that could improve tissue utilization rate by 1.52 fold, whereas it reduced the tissue-processing time by 80% compared with the traditional paraffin-embedding method. In animal studies, we demonstrated that the protocol would not affect the results of DNA mutation analysis. Furthermore, we explored the utility of this approach in non-small cell lung cancer because it is a compelling application for this innovation. We used 35 cases including 7 cases of biopsy specimens of non-small cell lung cancer to simulate future clinical application. The cascaded protocol consumed 150-μm thickness of formalin-fixed, paraffin-embedded specimens, providing 3D histologic and immunohistochemical information approximately 38 times that of the current paraffin-embedding protocol, and 3 rounds of DNA mutation analysis, offering both essential guidance for routine diagnostic evaluation and advanced information for precision medicine. Our designed integrated workflow provides an alternative way for pathological examination and paves the way for multidimensional tumor tissue assessment.
KW - Animals
KW - Humans
KW - Carcinoma, Non-Small-Cell Lung/genetics
KW - Lung Neoplasms/diagnostic imaging
KW - Quality of Life
KW - Mutation
KW - DNA
KW - Paraffin Embedding/methods
KW - Formaldehyde
U2 - 10.1016/j.labinv.2023.100195
DO - 10.1016/j.labinv.2023.100195
M3 - Article
C2 - 37302529
SN - 0023-6837
VL - 103
SP - 100195
JO - Laboratory Investigation
JF - Laboratory Investigation
IS - 9
ER -