Overexpression of ADP-ribosylation factor 1 in human gastric carcinoma and its clinicopathological significance

Ming Chieh Tsai; Paul Yann Lin; Wan Li Cheng; Chung Ying Tsai; Hsiang Cheng Chi; Cheng Yi Chen; Yi Hsin Tseng; Yi Fen Cheng; Dz Chi Chen; Ying Liang; Chia Jung Liao; Sheng Ming Wu; Yang Hsiang Lin; I. Hsiao Chung; Chia Siu Wang; Kwang Huei Lin

doi:10.1111/j.1349-7006.2012.02243.x

Overexpression of ADP-ribosylation factor 1 in human gastric carcinoma and its clinicopathological significance

Ming Chieh Tsai, Paul Yann Lin, Wan Li Cheng, Chung Ying Tsai, Hsiang Cheng Chi, Cheng Yi Chen, Yi Hsin Tseng, Yi Fen Cheng, Dz Chi Chen, Ying Liang, Chia Jung Liao, Sheng Ming Wu, Yang Hsiang Lin, I. Hsiao Chung, Chia Siu Wang, Kwang Huei Lin

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Gastric cancer is the sixth leading cause of cancer-related death in Taiwan, and the identification of related factors is essential to increase patient survival. ADP-ribosylation factor 1 (ARF1) was initially identified using 2-D electrophoresis combined with MALDI-time-of-flight mass spectrometry. ADP-ribosylation factor 1 belongs to the Ras superfamily or GTP-binding protein family and has been shown to enhance cell proliferation. In the current study, we evaluated the potential of ARF1 as a biomarker for gastric cancer detection. ADP-ribosylation factor 1 mRNA was upregulated in tumor tissues (compared with adjacent non-tumor tissues, n = 55) in approximately 67.2% of gastric cancer patients. Expression of ARF1 protein was additionally observed using Western blot and immunohistochemistry (IHC) analyses. The clinicopathological correlations of ARF1 were further evaluated. Elevated ARF1 expression was strongly correlated with lymph node metastasis (P = 0.008), serosal invasion (P = 0.046), lymphatic invasion (P = 0.035), and pathological staging (P = 0.010). Moreover, the 5-year survival rate for the lower ARF1 expression group (n = 50; IHC score < 90) was higher than that of the higher expression group (n = 60; IHC score ≥ 90) (P = 0.0228, log-rank test). To establish the specific function of ARF1 in human gastric cancer, isogenic ARF1-overexpressing cell lines were prepared. Our results showed that ARF1-overexpressing clones display enhanced cell proliferation, migration, and invasion. Furthermore, ARF1-overexpression might contribute to poor prognosis of patients. These findings collectively support the utility of ARF1 as a novel prognostic marker for gastric cancer and its role in cell invasion.

Original language	English
Pages (from-to)	1136-1144
Number of pages	9
Journal	Cancer Science
Volume	103
Issue number	6
DOIs	https://doi.org/10.1111/j.1349-7006.2012.02243.x
Publication status	Published - Jun 2012
Externally published	Yes

ASJC Scopus subject areas

Cancer Research
Oncology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1349-7006.2012.02243.x

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Tsai, M. C., Lin, P. Y., Cheng, W. L., Tsai, C. Y., Chi, H. C., Chen, C. Y., Tseng, Y. H., Cheng, Y. F., Chen, D. C., Liang, Y., Liao, C. J., Wu, S. M., Lin, Y. H., Chung, I. H., Wang, C. S., & Lin, K. H. (2012). Overexpression of ADP-ribosylation factor 1 in human gastric carcinoma and its clinicopathological significance. Cancer Science, 103(6), 1136-1144. https://doi.org/10.1111/j.1349-7006.2012.02243.x

Tsai, MC, Lin, PY, Cheng, WL, Tsai, CY, Chi, HC, Chen, CY, Tseng, YH, Cheng, YF, Chen, DC, Liang, Y, Liao, CJ, Wu, SM, Lin, YH, Chung, IH, Wang, CS & Lin, KH 2012, 'Overexpression of ADP-ribosylation factor 1 in human gastric carcinoma and its clinicopathological significance', Cancer Science, vol. 103, no. 6, pp. 1136-1144. https://doi.org/10.1111/j.1349-7006.2012.02243.x

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abstract = "Gastric cancer is the sixth leading cause of cancer-related death in Taiwan, and the identification of related factors is essential to increase patient survival. ADP-ribosylation factor 1 (ARF1) was initially identified using 2-D electrophoresis combined with MALDI-time-of-flight mass spectrometry. ADP-ribosylation factor 1 belongs to the Ras superfamily or GTP-binding protein family and has been shown to enhance cell proliferation. In the current study, we evaluated the potential of ARF1 as a biomarker for gastric cancer detection. ADP-ribosylation factor 1 mRNA was upregulated in tumor tissues (compared with adjacent non-tumor tissues, n = 55) in approximately 67.2% of gastric cancer patients. Expression of ARF1 protein was additionally observed using Western blot and immunohistochemistry (IHC) analyses. The clinicopathological correlations of ARF1 were further evaluated. Elevated ARF1 expression was strongly correlated with lymph node metastasis (P = 0.008), serosal invasion (P = 0.046), lymphatic invasion (P = 0.035), and pathological staging (P = 0.010). Moreover, the 5-year survival rate for the lower ARF1 expression group (n = 50; IHC score < 90) was higher than that of the higher expression group (n = 60; IHC score ≥ 90) (P = 0.0228, log-rank test). To establish the specific function of ARF1 in human gastric cancer, isogenic ARF1-overexpressing cell lines were prepared. Our results showed that ARF1-overexpressing clones display enhanced cell proliferation, migration, and invasion. Furthermore, ARF1-overexpression might contribute to poor prognosis of patients. These findings collectively support the utility of ARF1 as a novel prognostic marker for gastric cancer and its role in cell invasion.",

author = "Tsai, {Ming Chieh} and Lin, {Paul Yann} and Cheng, {Wan Li} and Tsai, {Chung Ying} and Chi, {Hsiang Cheng} and Chen, {Cheng Yi} and Tseng, {Yi Hsin} and Cheng, {Yi Fen} and Chen, {Dz Chi} and Ying Liang and Liao, {Chia Jung} and Wu, {Sheng Ming} and Lin, {Yang Hsiang} and Chung, {I. Hsiao} and Wang, {Chia Siu} and Lin, {Kwang Huei}",

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AU - Tsai, Ming Chieh

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AU - Cheng, Wan Li

AU - Tsai, Chung Ying

AU - Chi, Hsiang Cheng

AU - Chen, Cheng Yi

AU - Tseng, Yi Hsin

AU - Cheng, Yi Fen

AU - Chen, Dz Chi

AU - Liang, Ying

AU - Liao, Chia Jung

AU - Wu, Sheng Ming

AU - Lin, Yang Hsiang

AU - Chung, I. Hsiao

AU - Wang, Chia Siu

AU - Lin, Kwang Huei

PY - 2012/6

Y1 - 2012/6

N2 - Gastric cancer is the sixth leading cause of cancer-related death in Taiwan, and the identification of related factors is essential to increase patient survival. ADP-ribosylation factor 1 (ARF1) was initially identified using 2-D electrophoresis combined with MALDI-time-of-flight mass spectrometry. ADP-ribosylation factor 1 belongs to the Ras superfamily or GTP-binding protein family and has been shown to enhance cell proliferation. In the current study, we evaluated the potential of ARF1 as a biomarker for gastric cancer detection. ADP-ribosylation factor 1 mRNA was upregulated in tumor tissues (compared with adjacent non-tumor tissues, n = 55) in approximately 67.2% of gastric cancer patients. Expression of ARF1 protein was additionally observed using Western blot and immunohistochemistry (IHC) analyses. The clinicopathological correlations of ARF1 were further evaluated. Elevated ARF1 expression was strongly correlated with lymph node metastasis (P = 0.008), serosal invasion (P = 0.046), lymphatic invasion (P = 0.035), and pathological staging (P = 0.010). Moreover, the 5-year survival rate for the lower ARF1 expression group (n = 50; IHC score < 90) was higher than that of the higher expression group (n = 60; IHC score ≥ 90) (P = 0.0228, log-rank test). To establish the specific function of ARF1 in human gastric cancer, isogenic ARF1-overexpressing cell lines were prepared. Our results showed that ARF1-overexpressing clones display enhanced cell proliferation, migration, and invasion. Furthermore, ARF1-overexpression might contribute to poor prognosis of patients. These findings collectively support the utility of ARF1 as a novel prognostic marker for gastric cancer and its role in cell invasion.

AB - Gastric cancer is the sixth leading cause of cancer-related death in Taiwan, and the identification of related factors is essential to increase patient survival. ADP-ribosylation factor 1 (ARF1) was initially identified using 2-D electrophoresis combined with MALDI-time-of-flight mass spectrometry. ADP-ribosylation factor 1 belongs to the Ras superfamily or GTP-binding protein family and has been shown to enhance cell proliferation. In the current study, we evaluated the potential of ARF1 as a biomarker for gastric cancer detection. ADP-ribosylation factor 1 mRNA was upregulated in tumor tissues (compared with adjacent non-tumor tissues, n = 55) in approximately 67.2% of gastric cancer patients. Expression of ARF1 protein was additionally observed using Western blot and immunohistochemistry (IHC) analyses. The clinicopathological correlations of ARF1 were further evaluated. Elevated ARF1 expression was strongly correlated with lymph node metastasis (P = 0.008), serosal invasion (P = 0.046), lymphatic invasion (P = 0.035), and pathological staging (P = 0.010). Moreover, the 5-year survival rate for the lower ARF1 expression group (n = 50; IHC score < 90) was higher than that of the higher expression group (n = 60; IHC score ≥ 90) (P = 0.0228, log-rank test). To establish the specific function of ARF1 in human gastric cancer, isogenic ARF1-overexpressing cell lines were prepared. Our results showed that ARF1-overexpressing clones display enhanced cell proliferation, migration, and invasion. Furthermore, ARF1-overexpression might contribute to poor prognosis of patients. These findings collectively support the utility of ARF1 as a novel prognostic marker for gastric cancer and its role in cell invasion.

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Overexpression of ADP-ribosylation factor 1 in human gastric carcinoma and its clinicopathological significance

Abstract

ASJC Scopus subject areas

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