Over-expression of fibroblast growth factor receptor 3 in human hepatocellular carcinoma

Wei Hua Qiu, Bing Sen Zhou, Peiguo G. Chu, Wen Gang Chen, Christopher Chung, Jennifer Shih, Paul Hwu, Christopher Yeh, Richard Lopez, Yun Yen

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)


Aim: To describe the significant over-expression of fibroblast growth factor receptor 3 (FGFR3), which is a signal transduction and cell proliferation related gene in hepatocellular carcinoma (HCC). Methods: Following DNA microarray, Northern blot and quantitative real-time PCR were employed to confirm FGFR3 expression difference in HCC tissues and surrounding non-neoplastic liver tissue. FGFR3 expression levels were further determined by immunohistochemical study in 43 cases of HCC. Results: Northern blot results showed the significant over-expression of FGFR3 in HCC tissues, which was consistent with that from DNA microarray. Quantitative real-time PCR demonstrated that the mean ratio of FGFR3 mRNA to glyceraldehyde-3-phosphate dehydrogenase (GADPH) mRNA in HCC tissue was 0.250, whereas the ratio in non-neoplastic liver tissue was 0.014. Statistical analyses of 43 cases of HCC revealed that HCC scored higher than the matched non-neoplastic liver tissues. Examination of clinicopathological features revealed a strong correlation of over-expression of FGFR3 with poor tumor differentiation and high nuclear grade. Conclusion: Over-expression of FGFR3 may play an important role in liver carcinogenesis. FGFR3 may be an ideal candidate as a molecular marker in the diagnosis of HCC and a potential therapeutic target.

Original languageEnglish
Pages (from-to)5266-5272
Number of pages7
JournalWorld Journal of Gastroenterology
Issue number34
Publication statusPublished - Sept 14 2005
Externally publishedYes


  • Fibroblast growth factor receptor 3
  • Human hepatocellular carcinoma
  • Microarray

ASJC Scopus subject areas

  • Gastroenterology


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