Osteopontin Splicing Isoforms Contribute to Endometriotic Proliferation, Migration, and Epithelial-Mesenchymal Transition in Endometrial Epithelial Cells

Nguyen Tuong Ho, Shu Wei Lin, Yi Rong Lee, Chii Ruey Tzeng, Shu Huei Kao

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Osteopontin (OPN) isoforms, including OPNb and OPNc, promote malignancy and may contribute to the pathogenesis of endometriosis, a benign disorder with multiple characteristics resembling malignant tumors. In our experiments, OPNb and OPNc were significantly overexpressed in both endometriosis and adenomyosis compared to the normal endometrium. Upregulation of CD44v and the epithelial–mesenchymal transition (EMT) process was also present in endometriotic lesions. Overexpression of OPNb and OPNc splicing variants in endometriotic cells evoked morphological changes, actin remodeling, cell proliferation, cell migration, and EMT through binding OPN ligand receptors CD44 and αvβ3, subsequently activating the PI3K and NF-ĸB pathways. We elucidated the causal role of OPN splice variants in regulating endometriotic cell growth, which may promote the development of OPN-targeted therapies for patients suffering from endometriotic disorders.

Original languageEnglish
Article number15328
JournalInternational journal of molecular sciences
Volume23
Issue number23
DOIs
Publication statusPublished - Dec 2022

Keywords

  • CD44
  • endometriosis
  • epithelial-mesenchymal transition
  • migration
  • osteopontin splicing variants
  • proliferation
  • αvβ3

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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