@inbook{d9805208ca15465f851137147ed4bc68,
title = "Osteonectin promoter-mediated suicide gene therapy of prostate cancer",
abstract = "Suicide gene therapy using the herpes simplex virus thymidine kinase (HSV-tk) gene, combined with the prodrug ganciclovir (GCV) medication, is a promising approach for the treatment of malignant tumors, including prostate cancer. The success of this therapeutic strategy requires tissue- or tumor-specific gene expression and efficient gene delivery. In this chapter, we describe the experimental protocols of key methodologies, including promoter construction, reporter assay, adenoviral vector construction and preparation, HSV-tk enzymatic assay and cytotoxicity assay to evaluate the specificity and efficacy of osteonectin promoter-mediated HSV-tk/GCV suicide gene therapy of prostate cancer.",
keywords = "Adenoviral vector, HSV-tk enzymatic assay, Osteonectin promoter construction, Prostate cancer, Suicide gene therapy",
author = "Hsiao, {Wan Chi} and Sung, {Shian Ying} and Chung, {Leland W.K.} and Hsieh, {Chia Ling}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-8922-5_3",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "27--42",
booktitle = "Methods in Molecular Biology",
}
