Oral cancer plasma tumor marker identified with bead-based affinity-fractionated proteomic technology

Ann Joy Cheng; Li Chiu Chen; Kun Yi Chien; Yin Ju Chen; Joseph Tung Chieh Chang; Hung Ming Wang; Chun Ta Liao; I. How Chen

doi:10.1373/clinchem.2005.052324

Oral cancer plasma tumor marker identified with bead-based affinity-fractionated proteomic technology

Ann Joy Cheng, Li Chiu Chen, Kun Yi Chien, Yin Ju Chen, Joseph Tung Chieh Chang, Hung Ming Wang, Chun Ta Liao, I. How Chen

Research output: Contribution to journal › Article › peer-review

129 Citations (Scopus)

Abstract

Background: There is no plasma marker for detecting oral cancer, one of the most frequent cancers worldwide. We developed a bead-based affinity- fractionated proteomic method to discover a novel plasma marker for oral cancer. Methods: Affinity purification of heparinized plasma with magnetic beads and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis were used to screen potential oral cancer markers. We compiled MS protein profiles for 57 patients with oral cancer and compared them with profiles from 29 healthy controls. The spectra were analyzed statistically using flexAnalysis™ and Clin-Prot™ bioinformatic software. In each MS analysis, the peak intensities of interest were normalized with an internal standard (adrenocorticotropic hormone 18-39). For identification, affinity bead-purified plasma protein was subjected to MALDI TOF/TOF analysis followed by Mascot identification of the peptide sequences and a search of the National Center for Biotechnology Information protein database. Results: To optimize MALDI-TOF analysis based on the best discriminator of the cancer and control spectra, copper-chelated beads were used for plasma protein profiling. The within- and between-run CVs for assays were

Original language	English
Pages (from-to)	2236-2244
Number of pages	9
Journal	Clinical Chemistry
Volume	51
Issue number	12
DOIs	https://doi.org/10.1373/clinchem.2005.052324
Publication status	Published - Dec 2005
Externally published	Yes

ASJC Scopus subject areas

Clinical Biochemistry

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1373/clinchem.2005.052324

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title = "Oral cancer plasma tumor marker identified with bead-based affinity-fractionated proteomic technology",

abstract = "Background: There is no plasma marker for detecting oral cancer, one of the most frequent cancers worldwide. We developed a bead-based affinity- fractionated proteomic method to discover a novel plasma marker for oral cancer. Methods: Affinity purification of heparinized plasma with magnetic beads and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis were used to screen potential oral cancer markers. We compiled MS protein profiles for 57 patients with oral cancer and compared them with profiles from 29 healthy controls. The spectra were analyzed statistically using flexAnalysis{\texttrademark} and Clin-Prot{\texttrademark} bioinformatic software. In each MS analysis, the peak intensities of interest were normalized with an internal standard (adrenocorticotropic hormone 18-39). For identification, affinity bead-purified plasma protein was subjected to MALDI TOF/TOF analysis followed by Mascot identification of the peptide sequences and a search of the National Center for Biotechnology Information protein database. Results: To optimize MALDI-TOF analysis based on the best discriminator of the cancer and control spectra, copper-chelated beads were used for plasma protein profiling. The within- and between-run CVs for assays were ",

author = "Cheng, {Ann Joy} and Chen, {Li Chiu} and Chien, {Kun Yi} and Chen, {Yin Ju} and Chang, {Joseph Tung Chieh} and Wang, {Hung Ming} and Liao, {Chun Ta} and Chen, {I. How}",

year = "2005",

month = dec,

doi = "10.1373/clinchem.2005.052324",

language = "English",

volume = "51",

pages = "2236--2244",

journal = "Clinical Chemistry",

issn = "0009-9147",

publisher = "American Association for Clinical Chemistry Inc.",

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T1 - Oral cancer plasma tumor marker identified with bead-based affinity-fractionated proteomic technology

AU - Cheng, Ann Joy

AU - Chen, Li Chiu

AU - Chien, Kun Yi

AU - Chen, Yin Ju

AU - Chang, Joseph Tung Chieh

AU - Wang, Hung Ming

AU - Liao, Chun Ta

AU - Chen, I. How

PY - 2005/12

Y1 - 2005/12

N2 - Background: There is no plasma marker for detecting oral cancer, one of the most frequent cancers worldwide. We developed a bead-based affinity- fractionated proteomic method to discover a novel plasma marker for oral cancer. Methods: Affinity purification of heparinized plasma with magnetic beads and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis were used to screen potential oral cancer markers. We compiled MS protein profiles for 57 patients with oral cancer and compared them with profiles from 29 healthy controls. The spectra were analyzed statistically using flexAnalysis™ and Clin-Prot™ bioinformatic software. In each MS analysis, the peak intensities of interest were normalized with an internal standard (adrenocorticotropic hormone 18-39). For identification, affinity bead-purified plasma protein was subjected to MALDI TOF/TOF analysis followed by Mascot identification of the peptide sequences and a search of the National Center for Biotechnology Information protein database. Results: To optimize MALDI-TOF analysis based on the best discriminator of the cancer and control spectra, copper-chelated beads were used for plasma protein profiling. The within- and between-run CVs for assays were

AB - Background: There is no plasma marker for detecting oral cancer, one of the most frequent cancers worldwide. We developed a bead-based affinity- fractionated proteomic method to discover a novel plasma marker for oral cancer. Methods: Affinity purification of heparinized plasma with magnetic beads and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis were used to screen potential oral cancer markers. We compiled MS protein profiles for 57 patients with oral cancer and compared them with profiles from 29 healthy controls. The spectra were analyzed statistically using flexAnalysis™ and Clin-Prot™ bioinformatic software. In each MS analysis, the peak intensities of interest were normalized with an internal standard (adrenocorticotropic hormone 18-39). For identification, affinity bead-purified plasma protein was subjected to MALDI TOF/TOF analysis followed by Mascot identification of the peptide sequences and a search of the National Center for Biotechnology Information protein database. Results: To optimize MALDI-TOF analysis based on the best discriminator of the cancer and control spectra, copper-chelated beads were used for plasma protein profiling. The within- and between-run CVs for assays were

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JO - Clinical Chemistry

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Oral cancer plasma tumor marker identified with bead-based affinity-fractionated proteomic technology

Abstract

ASJC Scopus subject areas

UN SDGs

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