TY - JOUR
T1 - Optimal method for short-term or long-term islet preservation
T2 - comparison of islet culture, cold preservation and cryopreservation
AU - Liu, Fei
AU - Tian, Wencong
AU - Yang, Yinan
AU - Zhang, Qiong
AU - Zhu, Mengmeng
AU - Yang, Liang
AU - Yang, Lei
AU - Li, Jing
AU - Liu, Jie
AU - Wu, Ping
AU - Yang, Kaichiang
AU - Wang, Ximo
AU - Shen, Yanna
AU - Qi, Zhi
N1 - Publisher Copyright:
© 2014, The Japanese Society for Artificial Organs.
PY - 2014/12/5
Y1 - 2014/12/5
N2 - Islet preservation plays an important role for the success of islet transplantation. To determine the optimal method for islet preservation, we compared the outcomes of islet culture, cold preservation, and cryopreservation in this study. Isolated rat islets were divided into three groups: 37 °C group (conventional culture at 37 °C in RPMI-1640 medium), 4 °C group (cold preservation at 4 °C in University of Wisconsin (UW) solution), and −80 °C group (cryopreservation at −80 °C with dimethyl sulfoxide (DMSO)). Recovery rate, Calcein-AM/PI double staining, insulin release, mRNA level of hypoxia-inducible factor-1α (HIF-1α), and protein level of Bax in islets were examined after short-term (1 day) or long-term (7 days) preservation. After either short-term or long-term preservation, 4 °C group showed higher recovery rate of the islets number, lower percentage of PI positive area, better insulin release ability, and lower expression levels of HIF-1α and Bax in comparison to the 37 or −80 °C group. Meanwhile, islets in 37 °C group showed better function, and down-regulation of HIF-1α and Bax than those in −80 °C group on day 1; however, worse function of islets, up-regulated HIF-1α and Bax in 37 °C group were observed in comparison to −80 °C group on day 7. These results suggest that cold preservation at 4 °C in UW solution is the optimal method in comparison to the conventional culture at 37 °C or cryopreservation at −80 °C for short-term or long-term islet preservation. Furthermore, the potential mechanism may relate to, at least in part, apoptosis induced by the HIF-1α.
AB - Islet preservation plays an important role for the success of islet transplantation. To determine the optimal method for islet preservation, we compared the outcomes of islet culture, cold preservation, and cryopreservation in this study. Isolated rat islets were divided into three groups: 37 °C group (conventional culture at 37 °C in RPMI-1640 medium), 4 °C group (cold preservation at 4 °C in University of Wisconsin (UW) solution), and −80 °C group (cryopreservation at −80 °C with dimethyl sulfoxide (DMSO)). Recovery rate, Calcein-AM/PI double staining, insulin release, mRNA level of hypoxia-inducible factor-1α (HIF-1α), and protein level of Bax in islets were examined after short-term (1 day) or long-term (7 days) preservation. After either short-term or long-term preservation, 4 °C group showed higher recovery rate of the islets number, lower percentage of PI positive area, better insulin release ability, and lower expression levels of HIF-1α and Bax in comparison to the 37 or −80 °C group. Meanwhile, islets in 37 °C group showed better function, and down-regulation of HIF-1α and Bax than those in −80 °C group on day 1; however, worse function of islets, up-regulated HIF-1α and Bax in 37 °C group were observed in comparison to −80 °C group on day 7. These results suggest that cold preservation at 4 °C in UW solution is the optimal method in comparison to the conventional culture at 37 °C or cryopreservation at −80 °C for short-term or long-term islet preservation. Furthermore, the potential mechanism may relate to, at least in part, apoptosis induced by the HIF-1α.
KW - HIF-1α
KW - Islet
KW - Islet cold preservation
KW - Islet cryopreservation
KW - Islet culture
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U2 - 10.1007/s10047-014-0777-x
DO - 10.1007/s10047-014-0777-x
M3 - Article
C2 - 24944122
AN - SCOPUS:84914148779
SN - 1434-7229
VL - 17
SP - 337
EP - 343
JO - Journal of Artificial Organs
JF - Journal of Artificial Organs
IS - 4
ER -