Optical design of an LED lighting source for fluorescence microscopes

Tai Chih Kuo, Ting Jou Ding, Jui Hui Lin, Shih Hsin Ma

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


In this study, we reveal an LED light source model applied in fluorescence microscopes. This optical model is composed of a confocal total internal reflection lens array system (CTLAS) with a nine-LED array. The CTLAS optical system that we designed consists of a total internal reflection (TIR) lens array and a confocal system. The electrical power of the nine-LED array is 7.9 watts, which is lower than traditional light sources, such as the original 120-watt halogen lamps used in fluorescence microscopes (Zeiss, Axio Imager 2). We have successfully applied the CTLAS system to an Axio Imager 2 fluorescence microscope to observe the vascular bundle organization, modified with Cy3 fluorescence molecules, and have found that in the process of system assembly, the fabrication errors of optical lenses could have a critical effect on the CTLAS system. The results of our experiment show that, in order to achieve the same illuminance as that of the halogen lamp, the displacement error tolerances of the lateral x-axis and the longitudinal z-axis must be controlled within 1.3 mm and 1.7 mm, respectively.

Original languageEnglish
Article number4574
Pages (from-to)1-14
Number of pages14
JournalApplied Sciences (Switzerland)
Issue number21
Publication statusPublished - Nov 1 2019


  • LED light source
  • optical design
  • optical model
  • uniformity

ASJC Scopus subject areas

  • General Materials Science
  • Instrumentation
  • General Engineering
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes


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