Online RP-HPLC-DPPH screening method for detection of radical-scavenging phytochemicals from flowers of Acacia confusa

Jyh Horng Wu, Chih Yu Huang, Yu Tang Tung, Shang Tzen Chang

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76 Citations (Scopus)


Acacia confusa is traditionally used as a medicinal plant in Taiwan. In this study, phytochemicals and antioxidant activities of extracts from flowers of A. confusa were investigated for the first time. In addition, a rapid screening method, online RP-HPLC-DPPH system, for individual antioxidants in complex matrices was developed. Accordingly, six antioxidants including gallic acid (1), myricetin 3-rhamnoside (2), quercetin 3-rhamnoside (3), kaempferol 3-rhamnoside (4), europetin 3-rhamnoside (5), and rhamnetin 3-rhamnoside (6) were detected using the developed screening method. Of these, compounds 2, 3, and 5 were found to be major bioactive phytochemicals, and their contents were determined as 11.3, 6.7, and 8.7 mg/g of crude extract, respectively. By comparison with quercetin, a well-known antioxidant, these compounds had the order of compound 2 > compound 5 > quercetin > compound 3 for DPPH radical-scavenging activity. Their IC50 values were 3.0, 3.2, 4.5, and 7.4 μM, respectively. Moreover, the same order was observed for superoxide radical-scavenging activity, and their IC50 values were 2.6, 2.7, 4.3, and 5.3 μM, respectively. However, for lipid peroxidation, quercetin, an aglycon, showed the best inhibitory activity. The IC50 values of quercetin, compound 2, compound 5, and compound 3 were 46.7, 88.5, 90.7, and 124.6 μM, respectively. These results indicated that a rhamnoside at the C3 position of flavonoids had a negative effect on radical-scavenging activity and antilipid peroxidation. In contrast, the number of hydroxyl groups on the B-ring exhibited a positive relationship with their inhibitory activities.

Original languageEnglish
Pages (from-to)328-332
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Issue number2
Publication statusPublished - Jan 23 2008
Externally publishedYes


  • Acacia confusa
  • Anti-oxidant
  • Flower extract
  • Online RP-HPLC-DPPH
  • Radical-scavenging activity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)


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