@article{abacee7cc8a04ff2819b6dc852401e4b,
title = "Novel pd-l1 mab hc16 reveals upregulation of pd-l1 in bac subtype",
abstract = "Programmed death-ligand 1 (PD-L1) is an inhibitory transmembrane protein that can prevent autoimmune response. Upregulated PD-L1 serves as a predictive biomarker for patients who may respond well to immune checkpoint therapies. However, variable associations of PD-L1 level with prognoses have been report ed. In t hi s st udy, a short pept i de sequence corresponding to PD-L1 amino acids 172-187 (from the extracellular Ig-like C-type domain, and with high predicted antigenicity and hydrophilicity) was used to generate a monoclonal antibody (mAb). The resultant PD-L1 mAb, clone HC16, was examined for binding specificity and reactivity in cancer cell-lines, as assessed by immunocytochemical, immunoblotting, and co-immunoprecipitation. The potential diagnostic and clinical applicability of clone HC16 was further tested using malignant tissue arrays derived from various cancer t ypes anal yzed wi t h an aut omat ed i mmunohi st ochemi cal (I HC) st ai ni ng pl at f or m. Additionally, tumor samples from patients diagnosed with non-small cell lung cancer (NSCLC) were analyzed by western blotting. Clone HC16 showed obvious staining activity in lung and breast cancer tissues. Int erest i ngl y, we observed t hat PD-L1 l evel was negatively associated with clinical stage in NSCLC. Strong PD-L1 expression tended to be found in patients diagnosed with bronchioloalveolar carcinoma (BAC). These results demonstrate that clone HC16 harbors good target specificity and is suitable for further development in diagnostic tools to assess PD-L1 expression in human tissues. In addition, our findings also suggest a role for PD-L1 in a non-invasive subtype of lung cancer.",
keywords = "Monoclonal antibody, Non-small cell lung cancer (NSCLC), Programmed death-ligand 1",
author = "Su, {Bor Chyuan} and Ting, {Chen Hung} and Lee, {Kang Yun} and Wu, {Sheng Ming} and Feng, {Po Hao} and Chan, {Yao Fei} and Chen, {Jyh Yih}",
note = "Funding Information: We thank Dr. Marcus J. Calkins for language editing. We also thank Cyrusbioscience for the generation of PD-L1 mAb and Taipei Medical University, Shuang Ho Hospital in New Taipei City for providing the human samples from NSCLC patients. Funding. This work was financially supported by the iEGG and Animal Bi otechnol ogy Center from the Feature Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE-109-S-0023-A) in Taiwan. This work was partly financially supported by the 109-2313-B-001-007-MY3, 109-2622-B-001-002-CC1, or 108-2313-B-001-006?from the Ministry of Science and Technology (Taiwan) and a PI quota to Dr. Jyh-Yih Chen from the Marine Research Station, Institute of Cellular and Organismic Biology. This research was also funded by Taipei Medical University, grant number: TMU108-AE1-B22. The funders played no part in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Funding Information: Acknowledgements. We thank Dr. Marcus J. Calkins for language editing. We also thank Cyrusbioscience for the generation of PD-L1 mAb and Taipei Medical University, Shuang Ho Hospital in New Taipei City for providing the human samples from NSCLC patients. Funding. This work was financially supported by the iEGG and Animal Biotechnology Center from the Feature Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE-109-S-0023-A) in Taiwan. This work was partly financially supported by the 109-2313-B-001-007-MY3, 109-2622-B-001-002-CC1, or 108-2313-B-001-006—from the Ministry of Science and Technology (Taiwan) and a PI quota to Dr. Jyh-Yih Chen from the Marine Research Station, Institute of Cellular and Organismic Biology. This research was also funded by Taipei Medical University, grant number: TMU108-AE1-B22. The funders played no part in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Availability of data and materials. The materials and data generated and/or analyzed in this study are available from the corresponding author upon reasonable request. Authors' contributions. BC Su and CH Ting performed the experiments. KY Lee, SM Wu, PH Feng, and YF Chan designed the study. BC-Su, CH-Ting, and JY Chen wrote the manuscript. Ethics approval and consent to participate. The use of human tissue specimens was approved by the Institutional Review Board at Shuang Ho Hospital, Taipei Medical University (IRB No: N201702026). This study was conducted in accordance with the Declaration of Helsinki. Patient consent for publication. Not applicable. Competing interests. The authors have no competing financial interests to declare. Publisher Copyright: {\textcopyright} The Author(s) 2021.",
year = "2021",
month = jan,
doi = "10.14670/HH-18-272",
language = "English",
volume = "36",
pages = "77--89",
journal = "Histology and Histopathology",
issn = "0213-3911",
publisher = "Histology and Histopathology",
number = "1",
}