Novel ent-beyeran-19-oic acids from biotransformations of isosteviol metabolites by Mortierella isabellina

Che Ling Lin, Shwu Jiuan Lin, Wei Jan Huang, Yuan Ling Ku, Tung Hu Tsai, Feng-Lin Hsu

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10 Citations (Scopus)


Biotransformations of ent-16β-hydroxybeyeran-19-oic acid (1) by Mortierella isabellina produced hydroxylated metabolites. The isolated metabolites included three new compounds, ent-14β,16β- dihydroxybeyeran-19-oic acid (3), ent-12β-hydroxy-16-oxobeyeran-19-oic acid (4), and enr-7α,12β-dihydroxy-16-oxobeyeran-19-oic acid (5), and one known compound, ent-7α,16β-dihydroxybeyeran-19-oic acid (2). The structural elucidation was achieved by detailed analysis of LC-MS chromatograms, and MS and NMR spectroscopic data. In this study, M. isabellina hydroxylated the basic skeleton beyeran-19-oic acid at the 7β-, 12α-, and 14α-positions, and oxidized the skeleton at the 16-position. All compounds were evaluated with the cell viability assay. The results of the bioassay indicated that MTT formazan exocytosis occurs upon treatment of the cells with 1.

Original languageEnglish
Pages (from-to)1581-1587
Number of pages7
JournalPlanta Medica
Issue number15
Publication statusPublished - Dec 2007


  • ent-16β-Hydroxybeyeran-19-oic acid
  • Hydroxylation
  • Isoteviol
  • Microbial biotransformations
  • Mortierella isabellina
  • MTT formazan exocytosis
  • Stevioside

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology


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