Abstract
Mesona procumbens Hemseley is a well-known traditional herbal medicine used for heat-related ailments. In Taiwan, boiled extracts of M. procumbens are also used as desserts called grass jelly. In this study, the hexane extract from 75% EtOH of M. procumbens showed potent activities on inhibition of E. coli β-glucuronidase (eβG) and NO production and cytotoxicity against MCF-7 and HepG2 cancer cell lines. Furthermore, using various flash columns and HPLC chromatography on the bioactive layer led to the isolation of twelve compounds (1–12), including a new ent-kaurene, mesokaurol A (1), and a new germacrene derivative, mesogermapene A (2). Their structures were elucidated by extensive spectroscopic analyses, especially 2 D NMR and mass data. Biological assays showed that compound 9 (linolenic acid) had specific activity on inhibition of eβG (68.27%) at 100 µg/mL but was non-inhibitory to human β-glucuronidase. Compound 1 possessed significant cytotoxicity against MCF-7 (EC50 = 9.76 µM) and HepG2 (EC50 = 8.64 µM) cancer cell lines.
Original language | English |
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Pages (from-to) | 2172-2180 |
Number of pages | 9 |
Journal | Natural Product Research |
Volume | 37 |
Issue number | 13 |
DOIs | |
Publication status | Published - 2023 |
Keywords
- Anti-β-glucuronidase
- cytotoxicity
- germacrene
- Kaurane-type diterpene
- Mesona procumbens Hemseley
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Plant Science
- Organic Chemistry