Abstract
In continuing our investigation on the bioactive constituents ofmycelium of Antrodia camphorata, antroquinonol B (1), 4-acetyl-antroquinonol B (2), 2,3-(methylenedioxy)-6-methylbenzene-1,4-diol (3) and 2,4-dimethoxy-6- methylbenzene-1,3-diol (4) along with antrodin D (5) were isolated by the guidance of an inducible nitric oxide synthase (iNOS) inhibitory assay and identified on the basis of their spectroscopic analysis. The effect of these compounds on the inhibition of NO production in lipopolysaccharide (LPS)-activated murine macrophages was further evaluated. Compounds 4 and 5 significantly inhibited NO production without any cytotoxicity, the IC 50 values being 32.2 ± 0.1 and 26.3 ± 1.6 μ g/mL, respectively. Compounds 1 and 2 possessed greater effects on NO inhibition, with IC50 values of 16.2 ± 0.8 and 14.7 ± 2.8 μ g/mL, respectively, but displayed cytotoxicity at considerably higher concentrations. Compound 3 showed the lowest percent cell viability of 45.5 ± 1.8% as observed in treated cells at a concentration of 16.8 μ g/mL.
Original language | English |
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Pages (from-to) | 512-516 |
Number of pages | 5 |
Journal | Planta Medica |
Volume | 75 |
Issue number | 5 |
DOIs | |
Publication status | Published - Apr 2009 |
Keywords
- Antrodia camphorata
- Antroquinonol B
- iNOS
- Mycelium
- Polyporaceae
ASJC Scopus subject areas
- Complementary and alternative medicine
- Molecular Medicine
- Organic Chemistry
- Analytical Chemistry
- Pharmaceutical Science
- Pharmacology
- Drug Discovery