Neutralizing antibody provided protection against enterovirus type 71 lethal challenge in neonatal mice

Chun Keung Yu, Chi Chung Chen, Chi Long Chen, Jen Ren Wang, Ching Chuan Liu, Jing Jou Yan, Ih Jen Su

Research output: Contribution to journalArticlepeer-review

103 Citations (Scopus)


Experimental infection with enterovirus type 71 (EV71) induced death in neonatal mice in an age- and dose-dependent manner. The mortality rate was 100% following intraperitoneal inoculation 1-day-old ICR mice and this gradually decreased as the age at the time of inoculation increased (60% in 3-day-old mice and no deaths occurred in mice older than 6 days of age). A lethal dose greater than 108 PFU was necessary. Lethargy, failure to gain weight, rear limb tremors and paralysis were observed in the infected mice before death. EV71 was isolated from various tissues of the dead mice. Using a reverse transcription polymerase chain reaction technique with a specific primer pair, a 332-bp product was detected in the tissues that produced a culture positive for EV71. Protection against EV71 challenge in neonatal mice was demonstrated following passive transfer of serum from actively immunized adult mice 1 day after inoculation with the virus. Pups from hyperimmune dams were resistant to EV71 challenge. Additionally, maternal immunization with a formalin-inactivated whole-virus vaccine prolonged the survival of pups after EV71 lethal challenge. Copyright (C) 2000 National Science Council.

Original languageEnglish
Pages (from-to)523-528
Number of pages6
JournalJournal of Biomedical Science
Issue number6
Publication statusPublished - 2000
Externally publishedYes


  • Enterovirus type 71
  • Experimental infection
  • Mice
  • Neutralizing antibody
  • Vaccine

ASJC Scopus subject areas

  • Biochemistry, medical
  • Pharmacology (medical)
  • Molecular Biology
  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism
  • Cell Biology


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