Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation

Y. C. Huang; W. S. Tzeng; C. C. Wang; B. C. Cheng; Y. K. Chang; H. H. Chen; P. C. Lin; T. Y. Huang; T. J. Chuang; J. W. Lin; C. P. Chang

doi:10.1016/j.mri.2013.03.026

Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation

Y. C. Huang, W. S. Tzeng, C. C. Wang, B. C. Cheng, Y. K. Chang, H. H. Chen, P. C. Lin, T. Y. Huang, T. J. Chuang, J. W. Lin, C. P. Chang

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Purpose: This study aimed to further investigate the effects of agmatine on brain edema in the rats with middle cerebral artery occlusion (MCAO) injury using magnetic resonance imaging (MRI) monitoring and biochemical and histopathologic evaluation. Materials and methods: Following surgical induction of MCAO for 90. min, agmatine was injected 5. min after beginning of reperfusion and again once daily for the next 3 post-operative days. The events during ischemia and reperfusion were investigated by T2-weighted images (T2WI), serial diffusion-weighted images (DWI), calculated apparent diffusion coefficient (ADC) maps and contrast-enhanced T1-weighted images (CE-T1WI) during 3. h-72. h in a 1.5. T Siemens MAGNETON Avanto Scanner. Lesion volumes were analyzed in a blinded and randomized manner. Triphenyltetrazolium chloride (TTC), Nissl, and Evans Blue stainings were performed at the corresponding sections. Results: Increased lesion volumes derived from T2WI, DWI, ADC, CE-T1WI, and TTC all were noted at 3. h and peaked at 24. h-48. h after MCAO injury. TTC-derived infarct volumes were not significantly different from the T2WI, DWI-, and CE-T1WI-derived lesion volumes at the last imaging time (72. h) point except for significantly smaller ADC lesions in the MCAO model (P.

Original language	English
Pages (from-to)	1174-1181
Number of pages	8
Journal	Magnetic Resonance Imaging
Volume	31
Issue number	7
DOIs	https://doi.org/10.1016/j.mri.2013.03.026
Publication status	Published - Sept 2013

Keywords

Agmatine
Brain edema
Magnetic resonance image
Pharmacology
Stroke

ASJC Scopus subject areas

Biophysics
Radiology Nuclear Medicine and imaging
Biomedical Engineering

Access to Document

10.1016/j.mri.2013.03.026

Cite this

Huang, Y. C., Tzeng, W. S., Wang, C. C., Cheng, B. C., Chang, Y. K., Chen, H. H., Lin, P. C., Huang, T. Y., Chuang, T. J., Lin, J. W., & Chang, C. P. (2013). Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation. Magnetic Resonance Imaging, 31(7), 1174-1181. https://doi.org/10.1016/j.mri.2013.03.026

@article{c2717ea8598b4dc989052f739c945ba9,

title = "Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation",

abstract = "Purpose: This study aimed to further investigate the effects of agmatine on brain edema in the rats with middle cerebral artery occlusion (MCAO) injury using magnetic resonance imaging (MRI) monitoring and biochemical and histopathologic evaluation. Materials and methods: Following surgical induction of MCAO for 90. min, agmatine was injected 5. min after beginning of reperfusion and again once daily for the next 3 post-operative days. The events during ischemia and reperfusion were investigated by T2-weighted images (T2WI), serial diffusion-weighted images (DWI), calculated apparent diffusion coefficient (ADC) maps and contrast-enhanced T1-weighted images (CE-T1WI) during 3. h-72. h in a 1.5. T Siemens MAGNETON Avanto Scanner. Lesion volumes were analyzed in a blinded and randomized manner. Triphenyltetrazolium chloride (TTC), Nissl, and Evans Blue stainings were performed at the corresponding sections. Results: Increased lesion volumes derived from T2WI, DWI, ADC, CE-T1WI, and TTC all were noted at 3. h and peaked at 24. h-48. h after MCAO injury. TTC-derived infarct volumes were not significantly different from the T2WI, DWI-, and CE-T1WI-derived lesion volumes at the last imaging time (72. h) point except for significantly smaller ADC lesions in the MCAO model (P. ",

keywords = "Agmatine, Brain edema, Magnetic resonance image, Pharmacology, Stroke",

author = "Huang, {Y. C.} and Tzeng, {W. S.} and Wang, {C. C.} and Cheng, {B. C.} and Chang, {Y. K.} and Chen, {H. H.} and Lin, {P. C.} and Huang, {T. Y.} and Chuang, {T. J.} and Lin, {J. W.} and Chang, {C. P.}",

year = "2013",

month = sep,

doi = "10.1016/j.mri.2013.03.026",

language = "English",

volume = "31",

pages = "1174--1181",

journal = "Magnetic Resonance Imaging",

issn = "0730-725X",

publisher = "Elsevier Inc.",

number = "7",

}

TY - JOUR

T1 - Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation

AU - Huang, Y. C.

AU - Tzeng, W. S.

AU - Wang, C. C.

AU - Cheng, B. C.

AU - Chang, Y. K.

AU - Chen, H. H.

AU - Lin, P. C.

AU - Huang, T. Y.

AU - Chuang, T. J.

AU - Lin, J. W.

AU - Chang, C. P.

PY - 2013/9

Y1 - 2013/9

N2 - Purpose: This study aimed to further investigate the effects of agmatine on brain edema in the rats with middle cerebral artery occlusion (MCAO) injury using magnetic resonance imaging (MRI) monitoring and biochemical and histopathologic evaluation. Materials and methods: Following surgical induction of MCAO for 90. min, agmatine was injected 5. min after beginning of reperfusion and again once daily for the next 3 post-operative days. The events during ischemia and reperfusion were investigated by T2-weighted images (T2WI), serial diffusion-weighted images (DWI), calculated apparent diffusion coefficient (ADC) maps and contrast-enhanced T1-weighted images (CE-T1WI) during 3. h-72. h in a 1.5. T Siemens MAGNETON Avanto Scanner. Lesion volumes were analyzed in a blinded and randomized manner. Triphenyltetrazolium chloride (TTC), Nissl, and Evans Blue stainings were performed at the corresponding sections. Results: Increased lesion volumes derived from T2WI, DWI, ADC, CE-T1WI, and TTC all were noted at 3. h and peaked at 24. h-48. h after MCAO injury. TTC-derived infarct volumes were not significantly different from the T2WI, DWI-, and CE-T1WI-derived lesion volumes at the last imaging time (72. h) point except for significantly smaller ADC lesions in the MCAO model (P.

AB - Purpose: This study aimed to further investigate the effects of agmatine on brain edema in the rats with middle cerebral artery occlusion (MCAO) injury using magnetic resonance imaging (MRI) monitoring and biochemical and histopathologic evaluation. Materials and methods: Following surgical induction of MCAO for 90. min, agmatine was injected 5. min after beginning of reperfusion and again once daily for the next 3 post-operative days. The events during ischemia and reperfusion were investigated by T2-weighted images (T2WI), serial diffusion-weighted images (DWI), calculated apparent diffusion coefficient (ADC) maps and contrast-enhanced T1-weighted images (CE-T1WI) during 3. h-72. h in a 1.5. T Siemens MAGNETON Avanto Scanner. Lesion volumes were analyzed in a blinded and randomized manner. Triphenyltetrazolium chloride (TTC), Nissl, and Evans Blue stainings were performed at the corresponding sections. Results: Increased lesion volumes derived from T2WI, DWI, ADC, CE-T1WI, and TTC all were noted at 3. h and peaked at 24. h-48. h after MCAO injury. TTC-derived infarct volumes were not significantly different from the T2WI, DWI-, and CE-T1WI-derived lesion volumes at the last imaging time (72. h) point except for significantly smaller ADC lesions in the MCAO model (P.

KW - Agmatine

KW - Brain edema

KW - Magnetic resonance image

KW - Pharmacology

KW - Stroke

UR - http://www.scopus.com/inward/record.url?scp=84880731514&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84880731514&partnerID=8YFLogxK

U2 - 10.1016/j.mri.2013.03.026

DO - 10.1016/j.mri.2013.03.026

M3 - Article

C2 - 23642800

AN - SCOPUS:84880731514

SN - 0730-725X

VL - 31

SP - 1174

EP - 1181

JO - Magnetic Resonance Imaging

JF - Magnetic Resonance Imaging

IS - 7

ER -

Neuroprotective effect of agmatine in rats with transient cerebral ischemia using MR imaging and histopathologic evaluation

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this