NCAPG deregulation indicates poor patient survival and contributes to colorectal carcinogenesis

Ding Ping Sun; Chia Chun Wu; Chia Lin Chou; Li Chin Cheng; Wen Ching Wang; Shiau Shiuan Lin; Shih Ting Hung; Yu Feng Tian; Chia Lang Fang; Kai Yuan Lin

doi:10.1016/j.prp.2022.154238

NCAPG deregulation indicates poor patient survival and contributes to colorectal carcinogenesis

Ding Ping Sun, Chia Chun Wu, Chia Lin Chou, Li Chin Cheng, Wen Ching Wang, Shiau Shiuan Lin, Shih Ting Hung, Yu Feng Tian, Chia Lang Fang, Kai Yuan Lin

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Colorectal cancer (CRC) is one of the types of cancers with a high incidence and is ranked the 3rd among men and 2nd among women worldwide. The purpose of this study was to investigate the correlation between non-SMC condensin I complex subunit G (NCAPG) and the prognosis of CRC and its function in CRC cells. The expression of NCAPG in colorectal tissues and cells was detected by immunoblotting and immunohistochemistry. Kaplan–Meier analysis was used to analyze the correlation between NCAPG and CRC prognosis. RNAi technology was used to investigate how NCAPG inhibition affected the proliferation and migration of CRC cells. Overexpression of NCAPG was positively correlated with several clinicopathologic characteristics, including T stage (P = 0.0198), M stage (P = 0.0005), and TNM stage (P < 0.0001). Kaplan–Meier analysis showed that the overexpression of NCAPG was also negatively correlated with disease-free survival and overall survival. In the culture of CRC cells, the knockdown of NCAPG inhibited the proliferation, migration, and invasion of the cells. Meanwhile, it was also found that NCAPG knockdown could interfere with G2/M-G1 transition in the cell cycle, resulting in the inhibition of cell proliferation. The overexpression of NCAPG may serve as a candidate biomarker for CRC prognosis. NCAPG is also a potential therapeutic target for CRC.

Original language	English
Article number	154238
Journal	Pathology Research and Practice
Volume	241
DOIs	https://doi.org/10.1016/j.prp.2022.154238
Publication status	Published - Jan 2023

Keywords

Colorectal cancer
Invasion
Migration
NCAPG
Prognosis
Proliferation

ASJC Scopus subject areas

Pathology and Forensic Medicine
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.prp.2022.154238

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@article{ce2306238bc9423583938f28f3fcc57e,

title = "NCAPG deregulation indicates poor patient survival and contributes to colorectal carcinogenesis",

abstract = "Colorectal cancer (CRC) is one of the types of cancers with a high incidence and is ranked the 3rd among men and 2nd among women worldwide. The purpose of this study was to investigate the correlation between non-SMC condensin I complex subunit G (NCAPG) and the prognosis of CRC and its function in CRC cells. The expression of NCAPG in colorectal tissues and cells was detected by immunoblotting and immunohistochemistry. Kaplan–Meier analysis was used to analyze the correlation between NCAPG and CRC prognosis. RNAi technology was used to investigate how NCAPG inhibition affected the proliferation and migration of CRC cells. Overexpression of NCAPG was positively correlated with several clinicopathologic characteristics, including T stage (P = 0.0198), M stage (P = 0.0005), and TNM stage (P < 0.0001). Kaplan–Meier analysis showed that the overexpression of NCAPG was also negatively correlated with disease-free survival and overall survival. In the culture of CRC cells, the knockdown of NCAPG inhibited the proliferation, migration, and invasion of the cells. Meanwhile, it was also found that NCAPG knockdown could interfere with G2/M-G1 transition in the cell cycle, resulting in the inhibition of cell proliferation. The overexpression of NCAPG may serve as a candidate biomarker for CRC prognosis. NCAPG is also a potential therapeutic target for CRC.",

keywords = "Colorectal cancer, Invasion, Migration, NCAPG, Prognosis, Proliferation",

author = "Sun, {Ding Ping} and Wu, {Chia Chun} and Chou, {Chia Lin} and Cheng, {Li Chin} and Wang, {Wen Ching} and Lin, {Shiau Shiuan} and Hung, {Shih Ting} and Tian, {Yu Feng} and Fang, {Chia Lang} and Lin, {Kai Yuan}",

note = "Funding Information: This work was supported by two grants from Chi Mei Medical Center ( 109CM-TMU-03, CMFHR11130 ). The sponsor had no role in the collection, analysis and interpretation of data; in the writing of the manuscript; and in the decision to submit the manuscript for publication. The authors thank Crimson Interactive Pvt. Ltd. (Ulatus) for their assistance in manuscript translation and editing. Funding Information: This work was supported by two grants from Chi Mei Medical Center (109CM-TMU-03, CMFHR11130). The sponsor had no role in the collection, analysis and interpretation of data; in the writing of the manuscript; and in the decision to submit the manuscript for publication. The authors thank Crimson Interactive Pvt. Ltd. (Ulatus) for their assistance in manuscript translation and editing. Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2023",

month = jan,

doi = "10.1016/j.prp.2022.154238",

language = "English",

volume = "241",

journal = "Pathology Research and Practice",

issn = "0344-0338",

publisher = "Elsevier GmbH",

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TY - JOUR

T1 - NCAPG deregulation indicates poor patient survival and contributes to colorectal carcinogenesis

AU - Sun, Ding Ping

AU - Wu, Chia Chun

AU - Chou, Chia Lin

AU - Cheng, Li Chin

AU - Wang, Wen Ching

AU - Lin, Shiau Shiuan

AU - Hung, Shih Ting

AU - Tian, Yu Feng

AU - Fang, Chia Lang

AU - Lin, Kai Yuan

N1 - Funding Information: This work was supported by two grants from Chi Mei Medical Center ( 109CM-TMU-03, CMFHR11130 ). The sponsor had no role in the collection, analysis and interpretation of data; in the writing of the manuscript; and in the decision to submit the manuscript for publication. The authors thank Crimson Interactive Pvt. Ltd. (Ulatus) for their assistance in manuscript translation and editing. Funding Information: This work was supported by two grants from Chi Mei Medical Center (109CM-TMU-03, CMFHR11130). The sponsor had no role in the collection, analysis and interpretation of data; in the writing of the manuscript; and in the decision to submit the manuscript for publication. The authors thank Crimson Interactive Pvt. Ltd. (Ulatus) for their assistance in manuscript translation and editing. Publisher Copyright: © 2022 The Authors

PY - 2023/1

Y1 - 2023/1

N2 - Colorectal cancer (CRC) is one of the types of cancers with a high incidence and is ranked the 3rd among men and 2nd among women worldwide. The purpose of this study was to investigate the correlation between non-SMC condensin I complex subunit G (NCAPG) and the prognosis of CRC and its function in CRC cells. The expression of NCAPG in colorectal tissues and cells was detected by immunoblotting and immunohistochemistry. Kaplan–Meier analysis was used to analyze the correlation between NCAPG and CRC prognosis. RNAi technology was used to investigate how NCAPG inhibition affected the proliferation and migration of CRC cells. Overexpression of NCAPG was positively correlated with several clinicopathologic characteristics, including T stage (P = 0.0198), M stage (P = 0.0005), and TNM stage (P < 0.0001). Kaplan–Meier analysis showed that the overexpression of NCAPG was also negatively correlated with disease-free survival and overall survival. In the culture of CRC cells, the knockdown of NCAPG inhibited the proliferation, migration, and invasion of the cells. Meanwhile, it was also found that NCAPG knockdown could interfere with G2/M-G1 transition in the cell cycle, resulting in the inhibition of cell proliferation. The overexpression of NCAPG may serve as a candidate biomarker for CRC prognosis. NCAPG is also a potential therapeutic target for CRC.

AB - Colorectal cancer (CRC) is one of the types of cancers with a high incidence and is ranked the 3rd among men and 2nd among women worldwide. The purpose of this study was to investigate the correlation between non-SMC condensin I complex subunit G (NCAPG) and the prognosis of CRC and its function in CRC cells. The expression of NCAPG in colorectal tissues and cells was detected by immunoblotting and immunohistochemistry. Kaplan–Meier analysis was used to analyze the correlation between NCAPG and CRC prognosis. RNAi technology was used to investigate how NCAPG inhibition affected the proliferation and migration of CRC cells. Overexpression of NCAPG was positively correlated with several clinicopathologic characteristics, including T stage (P = 0.0198), M stage (P = 0.0005), and TNM stage (P < 0.0001). Kaplan–Meier analysis showed that the overexpression of NCAPG was also negatively correlated with disease-free survival and overall survival. In the culture of CRC cells, the knockdown of NCAPG inhibited the proliferation, migration, and invasion of the cells. Meanwhile, it was also found that NCAPG knockdown could interfere with G2/M-G1 transition in the cell cycle, resulting in the inhibition of cell proliferation. The overexpression of NCAPG may serve as a candidate biomarker for CRC prognosis. NCAPG is also a potential therapeutic target for CRC.

KW - Colorectal cancer

KW - Invasion

KW - Migration

KW - NCAPG

KW - Prognosis

KW - Proliferation

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DO - 10.1016/j.prp.2022.154238

M3 - Article

C2 - 36442414

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SN - 0344-0338

VL - 241

JO - Pathology Research and Practice

JF - Pathology Research and Practice

M1 - 154238

ER -

NCAPG deregulation indicates poor patient survival and contributes to colorectal carcinogenesis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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