Nanostructured titanium dioxide layer combined with reactive functional groups as a promising biofunctional surface for biomedical applications

Chi Ming Wu, Chung Ming Liu, Keng Liang Ou, Hsi Jen Chiang, Erwan Sugiatno, Chia Hung Wu, Hsiu Ju Yen, Hsin Hua Chou

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


In the present study, low-temperature plasma combined with allylamine polymerization approach was used to coat amino-groups on oxide layer of plasma-oxidized biomedical titanium (Ti) for protein immobilization. Scanning electron microscopy, X-ray diffractometer, transmission electron microscopy, X-ray photoemission spectroscopy, secondary ion mass spectrometer and 2, 4, 6-trinitrobenzenesulfonic acid assay were utilized to investigate the surface and microstructural properties of the plasma-oxidized, plasma-polymerized and protein-immobilized samples. Analytical results indicated that the presence of a nanostructured rutile-TiO 2 thin layer could be found on the plasma-oxidized samples. The thickness of nanostructured rutile-TiO 2 layer increased with increasing plasma treatment power and period. As the plasma-oxidized samples underwent plasma polymerization with allylamine, amino-groups (NH 2 ) were uniformly coated on nanostructured rutile-TiO 2 layer. It was also found that Ti surface with thick oxide layer exhibited higher amounts of amino-groups deposition. After protein immobilization, the plasma-polymerized samples presented a formation of uniform streak-like immobilized protein clusters. Therefore, biomedical Ti with nanostructured rutile-TiO 2 layer is a promising biomaterial that can be applied to cross-link with other biomolecules for promoting the bone healing and regeneration.

Original languageEnglish
Pages (from-to)9712-9718
Number of pages7
JournalCeramics International
Issue number8
Publication statusPublished - Jun 1 2019


  • Allylamine polymerization
  • Low-temperature plasma
  • Oxidation
  • Protein immobilization

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Process Chemistry and Technology
  • Surfaces, Coatings and Films
  • Materials Chemistry


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