TY - JOUR
T1 - Nanoformulation properties, characterization, and behavior in complex biological matrices
T2 - Challenges and opportunities for brain-targeted drug delivery applications and enhanced translational potential
AU - Agrahari, Vibhuti
AU - Burnouf, Pierre Alain
AU - Burnouf, Thierry
AU - Agrahari, Vivek
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Nanocarriers (synthetic/cell-based have attracted enormous interest for various therapeutic indications, including neurodegenerative disorders. A broader understanding of the impact of nanomedicines design is now required to enhance their translational potential. Nanoformulations in vivo journey is significantly affected by their physicochemical properties including the size, shape, hydrophobicity, elasticity, and surface charge/chemistry/morphology, which play a role as an interface with the biological environment. Understanding protein corona formation is crucial in characterizing nanocarriers and evaluating their interactions with biological systems. In this review, the types and properties of the brain-targeted nanocarriers are discussed. The biological factors and nanocarriers properties affecting their in vivo behavior are elaborated. The compositional description of cell culture and biological matrices, including proteins potentially relevant to protein corona built-up on nanoformulation especially for brain administration, is provided. Analytical techniques of characterizing nanocarriers in complex matrices, their advantages, limitations, and implementation challenges in industrial GMP environment are discussed. The uses of orthogonal complementary characterization approaches of nanocarriers are also covered.
AB - Nanocarriers (synthetic/cell-based have attracted enormous interest for various therapeutic indications, including neurodegenerative disorders. A broader understanding of the impact of nanomedicines design is now required to enhance their translational potential. Nanoformulations in vivo journey is significantly affected by their physicochemical properties including the size, shape, hydrophobicity, elasticity, and surface charge/chemistry/morphology, which play a role as an interface with the biological environment. Understanding protein corona formation is crucial in characterizing nanocarriers and evaluating their interactions with biological systems. In this review, the types and properties of the brain-targeted nanocarriers are discussed. The biological factors and nanocarriers properties affecting their in vivo behavior are elaborated. The compositional description of cell culture and biological matrices, including proteins potentially relevant to protein corona built-up on nanoformulation especially for brain administration, is provided. Analytical techniques of characterizing nanocarriers in complex matrices, their advantages, limitations, and implementation challenges in industrial GMP environment are discussed. The uses of orthogonal complementary characterization approaches of nanocarriers are also covered.
KW - Biological proteins
KW - Blood-brain barrier
KW - Cell-based nanocarriers, Neurodegenerative disorders
KW - Cell-culture media
KW - Characterization methods
KW - Nanoparticles
KW - Physicochemical properties
KW - Protein-corona
KW - Biological proteins
KW - Blood-brain barrier
KW - Cell-based nanocarrier
KW - Neurodegenerative disorders
KW - Cell-culture media
KW - Characterization methods
KW - Nanoparticles
KW - Physicochemical properties
KW - Protein-corona
UR - http://www.scopus.com/inward/record.url?scp=85062444425&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85062444425&partnerID=8YFLogxK
U2 - 10.1016/j.addr.2019.02.008
DO - 10.1016/j.addr.2019.02.008
M3 - Article
AN - SCOPUS:85062444425
SN - 0169-409X
VL - 148
SP - 146
EP - 180
JO - Advanced Drug Delivery Reviews
JF - Advanced Drug Delivery Reviews
ER -