TY - JOUR
T1 - Nanofiltration, a New Specific Virus Elimination Method Applied to High‐Purity Factor IX and Factor XI Concentrates
AU - Burnouf‐Radosevich, M.
AU - Appourchaux, P.
AU - Huart, J. J.
AU - Burnouf, T.
PY - 1994/8
Y1 - 1994/8
N2 - We have validated the use of two new regenerate multilayered structured cellulose membranes (BMM), Planova 15 N and Planova 35 N, with respective mean pore sizes of 15 and 35 nm, as a new filtration system to eliminate viruses in highly purified factor IX and factor XI concentrates. Virus spiking experiments indicated that single dead-end filtration on the membranes could remove more than 5.7-7.8 log10 of human immunodeficiency virus, bovine viral diarrhoea virus, porcine pseudorabies virus, reovirus type 3, and simian virus 40, as wall as the small non-enveloped viruses, poliovirus Sabin type 1 and bovine parvovirus. In vitro control tests and animal studies (Wessler stasis model, rat hypotension model) of the two concentrates did not reveal any significant differences with the non-nanofiltered material. Viral filtration of plasma derivatives on porous polymeric membranes might be an essential step in the improvement of their viral safety.
AB - We have validated the use of two new regenerate multilayered structured cellulose membranes (BMM), Planova 15 N and Planova 35 N, with respective mean pore sizes of 15 and 35 nm, as a new filtration system to eliminate viruses in highly purified factor IX and factor XI concentrates. Virus spiking experiments indicated that single dead-end filtration on the membranes could remove more than 5.7-7.8 log10 of human immunodeficiency virus, bovine viral diarrhoea virus, porcine pseudorabies virus, reovirus type 3, and simian virus 40, as wall as the small non-enveloped viruses, poliovirus Sabin type 1 and bovine parvovirus. In vitro control tests and animal studies (Wessler stasis model, rat hypotension model) of the two concentrates did not reveal any significant differences with the non-nanofiltered material. Viral filtration of plasma derivatives on porous polymeric membranes might be an essential step in the improvement of their viral safety.
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U2 - 10.1111/j.1423-0410.1994.tb01647.x
DO - 10.1111/j.1423-0410.1994.tb01647.x
M3 - Article
C2 - 7801601
AN - SCOPUS:0028095069
SN - 0042-9007
VL - 67
SP - 132
EP - 138
JO - Vox Sanguinis
JF - Vox Sanguinis
IS - 2
ER -
