NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion: A tool to improve transfusion safety

Sameh Obeid; Pei Shan Sung; Benoit Le Roy; Ming Li Chou; Shie Liang Hsieh; Celine Elie-Caille; Thierry Burnouf; Wilfrid Boireau

doi:10.1016/j.nano.2019.02.026

NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion: A tool to improve transfusion safety

Sameh Obeid, Pei Shan Sung, Benoit Le Roy, Ming Li Chou, Shie Liang Hsieh, Celine Elie-Caille, Thierry Burnouf, Wilfrid Boireau

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Plasma transfusion induces some transfusion related acute lung injury (TRALI) mediated through neutrophil extracellular traps (NETs). We investigated whether extracellular vesicles (EVs) present in plasma or obtained from resting (N-PEVs) or thrombin activated platelets (T-PEVs) can trigger NETs, and whether 75 nm-nanofiltration, to partially remove EVs, prohibits NETs formation. EVs size and concentration were determined by conventional biophysical approaches and by an original NanoBioAnalytical (NBA) platform based on EV immunocapture biochip, combining Surface Plasmon Resonance Imaging (SPRi) and Atomic Force Microscopy (AFM) exploration. EVs effective diameter was in the 25-1000 nm range, with a majority (≈ 90%) ≤ 100 nm. Both T-PEVs in buffer (but not N-PEVs) and non-nanofiltered plasma containing T-PEVs triggered NETs formation. Nanofiltration depleted large EVs (> 70 nm) and decreased NETs formation. The NBA platform was found to be a suitable tool to investigate the safety of plasma for transfusion.

Original language	English
Article number	101977
Pages (from-to)	101977
Journal	Nanomedicine: Nanotechnology, Biology, and Medicine
Volume	20
DOIs	https://doi.org/10.1016/j.nano.2019.02.026
Publication status	Published - Aug 1 2019

Keywords

AFM
Extra-cellular vesicles (EV)
NanoBioAnalytical platform
Nanofiltration
NETs
Plasma
Platelets
SPRi

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Molecular Medicine
Biomedical Engineering
General Materials Science
Pharmaceutical Science

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10.1016/j.nano.2019.02.026

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Obeid, S., Sung, P. S., Le Roy, B., Chou, M. L., Hsieh, S. L., Elie-Caille, C., Burnouf, T., & Boireau, W. (2019). NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion: A tool to improve transfusion safety. Nanomedicine: Nanotechnology, Biology, and Medicine, 20, 101977. Article 101977. https://doi.org/10.1016/j.nano.2019.02.026

Obeid, S, Sung, PS, Le Roy, B, Chou, ML, Hsieh, SL, Elie-Caille, C, Burnouf, T & Boireau, W 2019, 'NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion: A tool to improve transfusion safety', Nanomedicine: Nanotechnology, Biology, and Medicine, vol. 20, 101977, pp. 101977. https://doi.org/10.1016/j.nano.2019.02.026

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abstract = "Plasma transfusion induces some transfusion related acute lung injury (TRALI) mediated through neutrophil extracellular traps (NETs). We investigated whether extracellular vesicles (EVs) present in plasma or obtained from resting (N-PEVs) or thrombin activated platelets (T-PEVs) can trigger NETs, and whether 75 nm-nanofiltration, to partially remove EVs, prohibits NETs formation. EVs size and concentration were determined by conventional biophysical approaches and by an original NanoBioAnalytical (NBA) platform based on EV immunocapture biochip, combining Surface Plasmon Resonance Imaging (SPRi) and Atomic Force Microscopy (AFM) exploration. EVs effective diameter was in the 25-1000 nm range, with a majority (≈ 90%) ≤ 100 nm. Both T-PEVs in buffer (but not N-PEVs) and non-nanofiltered plasma containing T-PEVs triggered NETs formation. Nanofiltration depleted large EVs (> 70 nm) and decreased NETs formation. The NBA platform was found to be a suitable tool to investigate the safety of plasma for transfusion.",

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NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion: A tool to improve transfusion safety

Abstract

Keywords

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