Mutational events in LMP1 gene of epstein-barr virus in salivary gland lymphoepithelial carcinomas

Kai Yu Jen, Jun Cheng, Jian Li, Lanyan Wu, Yingfang Li, Shifeng Yu, Hanliang Lin, Zhilong Chen, Vladimir Gurtsevitch, Takashi Saku

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30 Citations (Scopus)


It is still unknown what kinds of roles Epstein-Barr virus (EBV) infection that are highly specific to salivary gland lymphoepithelial carcinomas (LECs) play in their tumorigenesis. To clarify the significance of EBV in LECs, we paid particular attention to the LMP1 gene, which is responsible for triggering several pathways for activating transcription factors. Sixty-one cases of EBV positive LECs confirmed by PCR and in-situ hybridization were collected from various areas of the world and studied immunohistochemically for latent membrane protein-1. Furthermore, PCR for the LMP1 carboxyl (C)-terminus region was performed, and the PCR products were sequenced for detection of other mutational events. LMP1 gene products were immunohistochemically demonstrated in 51% of the cases, while PCR amplification of the LMP1 gene was successful in 41 cases (67%). Among them, a 30 bp deletion in the C-terminus of the LMP1 gene, which had been shown to be characteristic to EBV in Chinese nasopharyngeal carcinomas, was found in 20 cases (32%). Most of them were from Guangzhou, Chengdu and Taiwan, while most of the cases from Shanghai and other areas exhibited no 30 bp deletion. In addition, several point mutations including codon 338 of LMP1 were commonly shared by the cases with or without the 30 bp deletion. These results indicate that there are 2 major genomic variations of EBV infecting salivary gland LECs. The frequent mutational events in the C-terminus in addition to the 30 bp deletion also seem to be critical for the pathogenesis because such mutational events may possibly promote cellular proliferation.

Original languageEnglish
Pages (from-to)654-660
Number of pages7
JournalInternational Journal of Cancer
Issue number5
Publication statusPublished - Jul 10 2003


  • EBV
  • LMP1
  • Lymphoepithelial carcinoma
  • Mutation
  • Salivary gland

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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