Multicolor ultra high throughput parallel microfluidic flow cytometer

Y. J. Fan; Yong Chen; Yi Chien Wu; Yu Chun Kung; Tinghsiang Wu; Horn Jiunn Sheen; Pei Yu Chiou

doi:10.1109/Transducers.2013.6627327

Multicolor ultra high throughput parallel microfluidic flow cytometer

Y. J. Fan, Yong Chen, Yi Chien Wu, Yu Chun Kung, Tinghsiang Wu, Horn Jiunn Sheen, Pei Yu Chiou

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

We report an ultrahigh throughput multi-color microfluidic flow cytometer with 32 detection channels and 64 sheath-flow channels. This flow cytometer is realized by integrating a high N.A. microball lens array with a 3D microfluidic device, which allows introducing cell samples into 32 sample channels, and sheath flows into 64 channel for parallel microfluidic focusing using only two inlets. A throughput of 384,000 cell/sec has been accomplished on a single device.

Original language	English
Title of host publication	2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Pages	2556-2559
Number of pages	4
DOIs	https://doi.org/10.1109/Transducers.2013.6627327
Publication status	Published - 2013
Externally published	Yes
Event	2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 - Barcelona, Spain Duration: Jun 16 2013 → Jun 20 2013

Conference

Conference	2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Country/Territory	Spain
City	Barcelona
Period	6/16/13 → 6/20/13

Keywords

BioMEMS
FACS
flow cytometry
microfluidics

ASJC Scopus subject areas

Hardware and Architecture
Electrical and Electronic Engineering

Access to Document

10.1109/Transducers.2013.6627327

Cite this

Fan, Y. J., Chen, Y., Wu, Y. C., Kung, Y. C., Wu, T., Sheen, H. J., & Chiou, P. Y. (2013). Multicolor ultra high throughput parallel microfluidic flow cytometer. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 (pp. 2556-2559). Article 6627327 https://doi.org/10.1109/Transducers.2013.6627327

Multicolor ultra high throughput parallel microfluidic flow cytometer. / Fan, Y. J.; Chen, Yong; Wu, Yi Chien et al.
2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 2556-2559 6627327.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Fan, YJ, Chen, Y, Wu, YC, Kung, YC, Wu, T, Sheen, HJ & Chiou, PY 2013, Multicolor ultra high throughput parallel microfluidic flow cytometer. in 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013., 6627327, pp. 2556-2559, 2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, Barcelona, Spain, 6/16/13. https://doi.org/10.1109/Transducers.2013.6627327

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title = "Multicolor ultra high throughput parallel microfluidic flow cytometer",

abstract = "We report an ultrahigh throughput multi-color microfluidic flow cytometer with 32 detection channels and 64 sheath-flow channels. This flow cytometer is realized by integrating a high N.A. microball lens array with a 3D microfluidic device, which allows introducing cell samples into 32 sample channels, and sheath flows into 64 channel for parallel microfluidic focusing using only two inlets. A throughput of 384,000 cell/sec has been accomplished on a single device.",

keywords = "BioMEMS, FACS, flow cytometry, microfluidics",

author = "Fan, {Y. J.} and Yong Chen and Wu, {Yi Chien} and Kung, {Yu Chun} and Tinghsiang Wu and Sheen, {Horn Jiunn} and Chiou, {Pei Yu}",

year = "2013",

doi = "10.1109/Transducers.2013.6627327",

language = "English",

isbn = "9781467359818",

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AU - Fan, Y. J.

AU - Chen, Yong

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AU - Sheen, Horn Jiunn

AU - Chiou, Pei Yu

PY - 2013

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N2 - We report an ultrahigh throughput multi-color microfluidic flow cytometer with 32 detection channels and 64 sheath-flow channels. This flow cytometer is realized by integrating a high N.A. microball lens array with a 3D microfluidic device, which allows introducing cell samples into 32 sample channels, and sheath flows into 64 channel for parallel microfluidic focusing using only two inlets. A throughput of 384,000 cell/sec has been accomplished on a single device.

AB - We report an ultrahigh throughput multi-color microfluidic flow cytometer with 32 detection channels and 64 sheath-flow channels. This flow cytometer is realized by integrating a high N.A. microball lens array with a 3D microfluidic device, which allows introducing cell samples into 32 sample channels, and sheath flows into 64 channel for parallel microfluidic focusing using only two inlets. A throughput of 384,000 cell/sec has been accomplished on a single device.

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KW - microfluidics

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BT - 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

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Y2 - 16 June 2013 through 20 June 2013

ER -

Multicolor ultra high throughput parallel microfluidic flow cytometer

Abstract

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Keywords

ASJC Scopus subject areas

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