TY - JOUR
T1 - Motorcycle exhaust particles augment antigen-induced airway inflammation in BALB/c mice
AU - Lee, Chen Chen
AU - Cheng, Yu Wen
AU - Liao, Jiunn Wang
AU - Chiang, Bor Luen
AU - Lai, Yih Loong
AU - Kang, Jaw Jou
PY - 2008/1
Y1 - 2008/1
N2 - Evidence indicates that environment pollutants from fossil fuel combustion compromise the immune system by enhancing allergic reactions and damaging the respiratory tract. This study was performed to investigate the effects of motorcycle exhaust particles (MEP), a major air pollutant especially in the urban areas of Taiwan, on allergen-induced airway inflammatory reactions in lab animals. BALB/c mice were intratracheally instilled with ovalbumin (OVA), MEP, or phosphate-buffered saline, 3 times every 2 wk. Airway hyperresponsiveness was measured in unrestrained mice by barometric plethsmography. Bronchoalveolar lavage fluid (BALF) and serum from treated animals were collected for cytokine and antibody determination by enzyme-linked immunosorbent assay (ELISA). Lung tissue stained with hematoxylin/eosin was examined. Data showed that MEP augmented OVA-induced airway inflammation; characterized by infiltration of eosinophils and neutrophils in BALF and lung tissue inflammation. The combination of OVA and MEP markedly increased interleukin-4 (IL-4), interleukin-5 (IL-5), and tumor necrosis factor-α (TNF-α) protein levels in BALF. In addition, MEP also augmented OVA-induced rise in OVA-specific immunoglobulin (Ig) G1 and IgE and airway hyperresponsiveness. Pretreated lavage cells with mitogen-activated protein kinase (MAPK) inhibitors showed that TNF-α release was significantly inhibited. This study found that MEP augmented antigen-induced allergic airway inflammation and airway hyperresponsiveness through a Th2-dominant pathway.
AB - Evidence indicates that environment pollutants from fossil fuel combustion compromise the immune system by enhancing allergic reactions and damaging the respiratory tract. This study was performed to investigate the effects of motorcycle exhaust particles (MEP), a major air pollutant especially in the urban areas of Taiwan, on allergen-induced airway inflammatory reactions in lab animals. BALB/c mice were intratracheally instilled with ovalbumin (OVA), MEP, or phosphate-buffered saline, 3 times every 2 wk. Airway hyperresponsiveness was measured in unrestrained mice by barometric plethsmography. Bronchoalveolar lavage fluid (BALF) and serum from treated animals were collected for cytokine and antibody determination by enzyme-linked immunosorbent assay (ELISA). Lung tissue stained with hematoxylin/eosin was examined. Data showed that MEP augmented OVA-induced airway inflammation; characterized by infiltration of eosinophils and neutrophils in BALF and lung tissue inflammation. The combination of OVA and MEP markedly increased interleukin-4 (IL-4), interleukin-5 (IL-5), and tumor necrosis factor-α (TNF-α) protein levels in BALF. In addition, MEP also augmented OVA-induced rise in OVA-specific immunoglobulin (Ig) G1 and IgE and airway hyperresponsiveness. Pretreated lavage cells with mitogen-activated protein kinase (MAPK) inhibitors showed that TNF-α release was significantly inhibited. This study found that MEP augmented antigen-induced allergic airway inflammation and airway hyperresponsiveness through a Th2-dominant pathway.
UR - http://www.scopus.com/inward/record.url?scp=38849123596&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38849123596&partnerID=8YFLogxK
U2 - 10.1080/15287390701801687
DO - 10.1080/15287390701801687
M3 - Article
C2 - 18246500
AN - SCOPUS:38849123596
SN - 1528-7394
VL - 71
SP - 405
EP - 412
JO - Journal of Toxicology and Environmental Health - Part A: Current Issues
JF - Journal of Toxicology and Environmental Health - Part A: Current Issues
IS - 6
ER -