Motility and protein phosphorylation in healthy and asthenozoospermic sperm

Chying Chyuan Chan; Hao Ai Shui; Ching Hsiang Wu; Chih Yuan Wang; Guang Huan Sun; Han Min Chen; Gwo Jang Wu

doi:10.1021/pr9003932

Motility and protein phosphorylation in healthy and asthenozoospermic sperm

Chying Chyuan Chan, Hao Ai Shui, Ching Hsiang Wu, Chih Yuan Wang, Guang Huan Sun, Han Min Chen, Gwo Jang Wu

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

Abstract

The majority of male infertility results from poor sperm motility. A direct link between altered protein phosphorylation and aberrant sperm motility has not been established. To address this issue, sperm samples obtained from 20 donors with healthy sperm and 20 donors with aberrantly motile sperm were subjected to computer assisted semen analysis (CASA), proteomic analysis, Western blot, and immunofluorescent staining. Proteomic analysis identified 12 protein spots as having differential phosphorylation, including gamma-tubulin complex associated protein 2 (GCP2). Western blot and immunofluorescence demonstrated differential expression of gamma-tubulin between healthy and aberrantly motile sperm. In conclusion, hypophosphorylated proteins and reduced expression of gamma-tubulin may be associated with low motility sperm.

Original language	English
Pages (from-to)	5382-5386
Number of pages	5
Journal	Journal of Proteome Research
Volume	8
Issue number	11
DOIs	https://doi.org/10.1021/pr9003932
Publication status	Published - Nov 6 2009
Externally published	Yes

Keywords

Gamma-tubulin
Protein phosphorylation
Sperm motility
Spermatozoa infertility

ASJC Scopus subject areas

Biochemistry
Chemistry(all)

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10.1021/pr9003932

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title = "Motility and protein phosphorylation in healthy and asthenozoospermic sperm",

abstract = "The majority of male infertility results from poor sperm motility. A direct link between altered protein phosphorylation and aberrant sperm motility has not been established. To address this issue, sperm samples obtained from 20 donors with healthy sperm and 20 donors with aberrantly motile sperm were subjected to computer assisted semen analysis (CASA), proteomic analysis, Western blot, and immunofluorescent staining. Proteomic analysis identified 12 protein spots as having differential phosphorylation, including gamma-tubulin complex associated protein 2 (GCP2). Western blot and immunofluorescence demonstrated differential expression of gamma-tubulin between healthy and aberrantly motile sperm. In conclusion, hypophosphorylated proteins and reduced expression of gamma-tubulin may be associated with low motility sperm.",

keywords = "Gamma-tubulin, Protein phosphorylation, Sperm motility, Spermatozoa infertility",

author = "Chan, {Chying Chyuan} and Shui, {Hao Ai} and Wu, {Ching Hsiang} and Wang, {Chih Yuan} and Sun, {Guang Huan} and Chen, {Han Min} and Wu, {Gwo Jang}",

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T1 - Motility and protein phosphorylation in healthy and asthenozoospermic sperm

AU - Chan, Chying Chyuan

AU - Shui, Hao Ai

AU - Wu, Ching Hsiang

AU - Wang, Chih Yuan

AU - Sun, Guang Huan

AU - Chen, Han Min

AU - Wu, Gwo Jang

PY - 2009/11/6

Y1 - 2009/11/6

N2 - The majority of male infertility results from poor sperm motility. A direct link between altered protein phosphorylation and aberrant sperm motility has not been established. To address this issue, sperm samples obtained from 20 donors with healthy sperm and 20 donors with aberrantly motile sperm were subjected to computer assisted semen analysis (CASA), proteomic analysis, Western blot, and immunofluorescent staining. Proteomic analysis identified 12 protein spots as having differential phosphorylation, including gamma-tubulin complex associated protein 2 (GCP2). Western blot and immunofluorescence demonstrated differential expression of gamma-tubulin between healthy and aberrantly motile sperm. In conclusion, hypophosphorylated proteins and reduced expression of gamma-tubulin may be associated with low motility sperm.

AB - The majority of male infertility results from poor sperm motility. A direct link between altered protein phosphorylation and aberrant sperm motility has not been established. To address this issue, sperm samples obtained from 20 donors with healthy sperm and 20 donors with aberrantly motile sperm were subjected to computer assisted semen analysis (CASA), proteomic analysis, Western blot, and immunofluorescent staining. Proteomic analysis identified 12 protein spots as having differential phosphorylation, including gamma-tubulin complex associated protein 2 (GCP2). Western blot and immunofluorescence demonstrated differential expression of gamma-tubulin between healthy and aberrantly motile sperm. In conclusion, hypophosphorylated proteins and reduced expression of gamma-tubulin may be associated with low motility sperm.

KW - Gamma-tubulin

KW - Protein phosphorylation

KW - Sperm motility

KW - Spermatozoa infertility

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Motility and protein phosphorylation in healthy and asthenozoospermic sperm

Abstract

Keywords

ASJC Scopus subject areas

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