Molecular imaging of enhanced Na+ expression in the liver of total sleep deprived rats by TOF-SIMS

Hung Ming Chang, Bo Jung Chen, Un In Wu, Yi Lun Huang, Fu Der Mai

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Sleep disorder is associated with metabolic disturbances, which was related to oxidative stress and subsequently sodium overload. Since liver plays important roles in metabolic regulation, present study is aimed to determine whether hepatic sodium, together with oxidative stress, would significantly alter after total sleep deprivation (TSD). Sodium ion was investigated by time-of-flight secondary ion mass spectrometry (TOF-SIMS). Parameter for oxidative stress was examined by heat shock protein-25 (HSP-25) immunohistochemistry. TOF-SIMS spectrum indicated that hepatic Na+/K+ ratio counting as 82.41 ± 9.5 was obtained in normal rats. Sodium ions were distributed in hepatocytes with several aggregations. However, following TSD, the intensity for Na+/K+ ratio was relatively increased (101.94 ± 6.9) and signals for sodium image were strongly expressed throughout hepatocytes without spatial localization. Quantitative analysis revealed that HSP-25 staining intensity is 1.78 ± 0.27 in TSD rats, which was significantly higher than that of normal ones (0.68 ± 0.15). HSP-25 augmentation suggests that hepatocytes suffer from oxidative stress following TSD. Concerning oxidative stress induced sodium overload would impair metabolic function; enhanced hepatic sodium expression after TSD may be a major cause of TSD relevant metabolic diseases.

Original languageEnglish
Pages (from-to)1131-1134
Number of pages4
JournalApplied Surface Science
Issue number4
Publication statusPublished - Dec 15 2008


  • Hepatocyte
  • Image analysis
  • Oxidative stress
  • Sleep deprivation
  • Sodium

ASJC Scopus subject areas

  • Surfaces, Coatings and Films


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