Molecular detection of rifabutin-susceptible Mycobacterium tuberculosis

Huang Yao Chen; Ming Chih Yu; Wei Lun Huang; Mei Hua Wu; Yung Lin Chang; Chien Rai Che; Ruwen Jou

doi:10.1128/JCM.00652-12

Molecular detection of rifabutin-susceptible Mycobacterium tuberculosis

Huang Yao Chen, Ming Chih Yu, Wei Lun Huang, Mei Hua Wu, Yung Lin Chang, Chien Rai Che, Ruwen Jou

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Rapid assays are still needed to detect rifabutin (RFB) susceptibility for proper tuberculosis treatment. To assess the use of the GenoType MTBDRplus assay and subsequent rpoB gene sequencing on detection of RFB susceptibility, we analyzed 800 multidrug- resistant Mycobacterium tuberculosis isolates, and 13% (104/800) were RFB susceptible. Of the 104 RFB-susceptible isolates, 71 (68.3%) isolates were rapidly identified using two molecular assays, while the remaining isolates could be determined using conventional drug-susceptibility testing according to the clinician's decision.

Original language	English
Pages (from-to)	2085-2088
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	50
Issue number	6
DOIs	https://doi.org/10.1128/JCM.00652-12
Publication status	Published - Jun 2012

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/JCM.00652-12

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title = "Molecular detection of rifabutin-susceptible Mycobacterium tuberculosis",

abstract = "Rapid assays are still needed to detect rifabutin (RFB) susceptibility for proper tuberculosis treatment. To assess the use of the GenoType MTBDRplus assay and subsequent rpoB gene sequencing on detection of RFB susceptibility, we analyzed 800 multidrug- resistant Mycobacterium tuberculosis isolates, and 13% (104/800) were RFB susceptible. Of the 104 RFB-susceptible isolates, 71 (68.3%) isolates were rapidly identified using two molecular assays, while the remaining isolates could be determined using conventional drug-susceptibility testing according to the clinician's decision.",

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AU - Chen, Huang Yao

AU - Yu, Ming Chih

AU - Huang, Wei Lun

AU - Wu, Mei Hua

AU - Chang, Yung Lin

AU - Che, Chien Rai

AU - Jou, Ruwen

PY - 2012/6

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N2 - Rapid assays are still needed to detect rifabutin (RFB) susceptibility for proper tuberculosis treatment. To assess the use of the GenoType MTBDRplus assay and subsequent rpoB gene sequencing on detection of RFB susceptibility, we analyzed 800 multidrug- resistant Mycobacterium tuberculosis isolates, and 13% (104/800) were RFB susceptible. Of the 104 RFB-susceptible isolates, 71 (68.3%) isolates were rapidly identified using two molecular assays, while the remaining isolates could be determined using conventional drug-susceptibility testing according to the clinician's decision.

AB - Rapid assays are still needed to detect rifabutin (RFB) susceptibility for proper tuberculosis treatment. To assess the use of the GenoType MTBDRplus assay and subsequent rpoB gene sequencing on detection of RFB susceptibility, we analyzed 800 multidrug- resistant Mycobacterium tuberculosis isolates, and 13% (104/800) were RFB susceptible. Of the 104 RFB-susceptible isolates, 71 (68.3%) isolates were rapidly identified using two molecular assays, while the remaining isolates could be determined using conventional drug-susceptibility testing according to the clinician's decision.

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Molecular detection of rifabutin-susceptible Mycobacterium tuberculosis

Abstract

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