Molecular characterization of enrofloxacin resistant Actinobacillus pleuropneumoniae isolates

Yu Chih Wang; Jacky Peng Wen Chan; Kuang Sheng Yeh; Chao Chin Chang; Shih Ling Hsuan; Yi Mei Hsieh; Yi Chih Chang; Tan Chen Lai; Wen Hsin Lin; Ter Hsin Chen

doi:10.1016/j.vetmic.2009.09.067

Molecular characterization of enrofloxacin resistant Actinobacillus pleuropneumoniae isolates

Yu Chih Wang, Jacky Peng Wen Chan, Kuang Sheng Yeh, Chao Chin Chang, Shih Ling Hsuan, Yi Mei Hsieh, Yi Chih Chang, Tan Chen Lai, Wen Hsin Lin, Ter Hsin Chen

Department of Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Enrofloxacin (ER) resistant Actinobacillus pleuropneumoniae strains emerged in Taiwan in 2002. The mechanism of ER resistance in A. pleuropneumoniae has not yet been reported. A total of 48 A. pleuropneumoniae isolates were obtained from the lungs of pigs with pleuropneumonia in Taiwan between September 2007 and April 2008. Twenty-nine isolates were found to be resistant to enrofloxacin. To understand the mechanisms of A. pleuropneumoniae's resistance to ER, enrofloxacin susceptibility of the isolates along with the mutations of the quinolone resistance-determining region (QRDR) of DNA gyrase and topoisomerase IV, qnr genes were analyzed. Enrofloxacin resistant isolates were found to carry at least one mutation in the QRDR of gyrA, leading to amino acid changes at codon 83 or 87. Efflux pump inhibitor (Phe-Arg-β-naphthylamide) decreased enrofloxacin minimum inhibitory concentration 2-16-fold, suggesting participation of efflux in ER resistance. Plasmid mediated quinolone resistance genes qnr were not detected in these isolates. In conclusion, enrofloxacin resistance of A. pleuropneumoniae may be linked to multiple target gene mutations and active effluxs.

Original language	English
Pages (from-to)	309-312
Number of pages	4
Journal	Veterinary Microbiology
Volume	142
Issue number	3-4
DOIs	https://doi.org/10.1016/j.vetmic.2009.09.067
Publication status	Published - May 2010

Keywords

Actinobacillus pleuropneumoniae
Efflux pump
Enrofloxacin resistance
Quinolone resistance-determining region

ASJC Scopus subject areas

Microbiology
General Veterinary

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10.1016/j.vetmic.2009.09.067

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title = "Molecular characterization of enrofloxacin resistant Actinobacillus pleuropneumoniae isolates",

abstract = "Enrofloxacin (ER) resistant Actinobacillus pleuropneumoniae strains emerged in Taiwan in 2002. The mechanism of ER resistance in A. pleuropneumoniae has not yet been reported. A total of 48 A. pleuropneumoniae isolates were obtained from the lungs of pigs with pleuropneumonia in Taiwan between September 2007 and April 2008. Twenty-nine isolates were found to be resistant to enrofloxacin. To understand the mechanisms of A. pleuropneumoniae's resistance to ER, enrofloxacin susceptibility of the isolates along with the mutations of the quinolone resistance-determining region (QRDR) of DNA gyrase and topoisomerase IV, qnr genes were analyzed. Enrofloxacin resistant isolates were found to carry at least one mutation in the QRDR of gyrA, leading to amino acid changes at codon 83 or 87. Efflux pump inhibitor (Phe-Arg-β-naphthylamide) decreased enrofloxacin minimum inhibitory concentration 2-16-fold, suggesting participation of efflux in ER resistance. Plasmid mediated quinolone resistance genes qnr were not detected in these isolates. In conclusion, enrofloxacin resistance of A. pleuropneumoniae may be linked to multiple target gene mutations and active effluxs.",

keywords = "Actinobacillus pleuropneumoniae, Efflux pump, Enrofloxacin resistance, Quinolone resistance-determining region",

author = "Wang, {Yu Chih} and Chan, {Jacky Peng Wen} and Yeh, {Kuang Sheng} and Chang, {Chao Chin} and Hsuan, {Shih Ling} and Hsieh, {Yi Mei} and Chang, {Yi Chih} and Lai, {Tan Chen} and Lin, {Wen Hsin} and Chen, {Ter Hsin}",

year = "2010",

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doi = "10.1016/j.vetmic.2009.09.067",

language = "English",

volume = "142",

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T1 - Molecular characterization of enrofloxacin resistant Actinobacillus pleuropneumoniae isolates

AU - Wang, Yu Chih

AU - Chan, Jacky Peng Wen

AU - Yeh, Kuang Sheng

AU - Chang, Chao Chin

AU - Hsuan, Shih Ling

AU - Hsieh, Yi Mei

AU - Chang, Yi Chih

AU - Lai, Tan Chen

AU - Lin, Wen Hsin

AU - Chen, Ter Hsin

PY - 2010/5

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AB - Enrofloxacin (ER) resistant Actinobacillus pleuropneumoniae strains emerged in Taiwan in 2002. The mechanism of ER resistance in A. pleuropneumoniae has not yet been reported. A total of 48 A. pleuropneumoniae isolates were obtained from the lungs of pigs with pleuropneumonia in Taiwan between September 2007 and April 2008. Twenty-nine isolates were found to be resistant to enrofloxacin. To understand the mechanisms of A. pleuropneumoniae's resistance to ER, enrofloxacin susceptibility of the isolates along with the mutations of the quinolone resistance-determining region (QRDR) of DNA gyrase and topoisomerase IV, qnr genes were analyzed. Enrofloxacin resistant isolates were found to carry at least one mutation in the QRDR of gyrA, leading to amino acid changes at codon 83 or 87. Efflux pump inhibitor (Phe-Arg-β-naphthylamide) decreased enrofloxacin minimum inhibitory concentration 2-16-fold, suggesting participation of efflux in ER resistance. Plasmid mediated quinolone resistance genes qnr were not detected in these isolates. In conclusion, enrofloxacin resistance of A. pleuropneumoniae may be linked to multiple target gene mutations and active effluxs.

KW - Actinobacillus pleuropneumoniae

KW - Efflux pump

KW - Enrofloxacin resistance

KW - Quinolone resistance-determining region

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Molecular characterization of enrofloxacin resistant Actinobacillus pleuropneumoniae isolates

Abstract

Keywords

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