MMP-13 is involved in oral cancer cell metastasis

Shun Hong Huang, Ching Hsuan Law, Ping Hsueh Kuo, Ren Yu Hu, Ching Chieh Yang, Ting Wen Chung, Ji Min Li, Li Hsun Lin, Yi Chung Liu, En Chi Liao, Yi Ting Tsai, Yu Shan Wei, Chi Chen Lin, Chien Wen Chang, Hsiu Chuan Chou, Wen Ching Wang, Margaret Dah Tsyr Chang, Lu Hai Wang, Hsing Jien Kung, Hong Lin ChanPing Chiang Lyu

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


The oral cancer cell line OC3-I5 with a highly invasive ability was selected and derived from an established OSCC line OC3. In this study, we demonstrated that matrix metalloproteinases protein MMP-13 was up-regulated in OC3-I5 than in OC3 cells. We also observed that expression of epithelial-mesenchymal transition (EMT) markers including Twist, p-Src, Snail1, SIP1, JAM-A, and vinculin were increased in OC3-I5 compared to OC3 cells, whereas E-cadherin expression was decreased in the OC3-I5 cells. Using siMMP-13 knockdown techniques, we showed that siMMP-13 not only reduced the invasion and migration, but also the adhesion abilities of oral cancer cells. In support of the role of MMP-13 in metastasis, we used MMP-13 expressing plasmid-transfected 293T cells to enhance MMP-13 expression in the OC3 cells, transplanting the MMP-13 over expressing OC3 cells into nude mice led to enhanced lung metastasis. In summary, our findings show that MMP-13 promotes invasion and metastasis in oral cancer cells, suggesting altered expression of MMP-13 may be utilized to impede the process of metastasis.

Original languageEnglish
Pages (from-to)17144-17161
Number of pages18
Issue number13
Publication statusPublished - Mar 29 2016
Externally publishedYes


  • ECM
  • EMT
  • IF
  • MMP13
  • OSCC

ASJC Scopus subject areas

  • Oncology


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